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General Information
Symbol
Dmel\lokKD
Species
D. melanogaster
Name
FlyBase ID
FBal0325825
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

A20063393C

Reported nucleotide change:

A?C

Amino acid change:

D303A | lok-PA; D286A | lok-PB; D286A | lok-PC

Reported amino acid change:

D286A

Comment:

Residue 286 relative to lok-PB was mutated from GCC to GAC (A286D).

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: D286A.

The D286A amino acid replacement (responsible for CHK2 kinase activity) results in a kinase-dead lok.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

lokKD homo- and heterozygous as well as lokKD/lokp6 females show a much milder loss of germline stem cells upon X-ray irradiation than wild-type controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

lok[+]/lokKD is an enhancer of increased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

Suppressor of
Statement
Reference

lok[+]/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokp6/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokp6/lokKD is a suppressor of increased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokKD/lokKD is a suppressor of decreased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokKD/lokKD is a suppressor of increased cell number | female | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

Other
Phenotype Manifest In
Enhancer of
Statement
Reference

lok[+]/lokKD is an enhancer of cystoblast | supernumerary | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

Suppressor of
Statement
Reference

lok[+]/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokp6/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokp6/lokKD is a suppressor of cystoblast | supernumerary | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokKD/lokKD is a suppressor of female germline stem cell | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

lokKD/lokKD is a suppressor of cystoblast | supernumerary | adult stage | temperature conditional phenotype of Crei\I-CreIhs.PR

Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The loss of germline stem cells (GSCs) observed upon heat-shock induced expression of Crei\I-CreIhs.PR (to introduce DNA damage) is rescued by combination with lokp6 or lokKD in hetero-, homo- or transheterozygosity as these double mutant flies contain significantly higher number of GSCs both at 3 days and 1 week post heat-shock compared to Crei\I-CreIhs.PR single mutants.

lokKD/+;Crei\I-CreIhs.PR, lokKD/lokKD;Crei\I-CreIhs.PR or lokKD/lokp6;Crei\I-CreIhs.PR mutants have more GSCs than wild-type controls even without the induction of DNA damage by heat-shock.

Crei\I-CreIhs.PR germaria in which DNA damage has been evoked by heat-shock accumulate cystoblast-like single cells and this accumulation is accelerated by lokp6 or lokKD heterozygosity (significantly increased number of CB-like cells observed already at 3 days post heat-shock). By contrast, both lokp6 or lokKD homo- or transheterozygosity completely abolishes the CB-like cell accumulation phenotype.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (1)