FB2025_01 , released February 20, 2025
Allele: Dmel\Stimko
Open Close
General Information
Symbol
Dmel\Stimko
Species
D. melanogaster
Name
FlyBase ID
FBal0326137
Feature type
allele
Associated gene
Dmel\Stim
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Pathak et al., 2017)
Mutagen
CRISPR/Cas9
(Pathak et al., 2017)
Progenitor genotype
Cytology
Description

Deletion of the Stim locus.

(Pathak et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Stimko homozygotes are consistently smaller throughout larval development which is significantly delayed relative to controls and the animals die as late second or early third instar larvae.

      Stimko heterozygotes show developmental and eclosion rate that is comparable to wild-type.

      (Pathak et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Other
      Statement
      Reference

      olf186-F[+]/OraiEY09167, Stimko has partially lethal - majority live phenotype

      (Pathak et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Stimko/+;olf186-FEY09167/+ double heterozygotes show reduced eclosion rate as significantly fewer adults emerge compared to wild-type and the single heterozygotes.

      (Pathak et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Stimko is rescued by Scer\GAL4Act5C.PI/StimUAS.cAa

      (Pathak et al., 2017)
      Partially rescued by

      Stimko is partially rescued by StimUAS.cAa/Scer\GAL4nSyb.PS

      (Pathak et al., 2017)

      Stimko is partially rescued by Scer\GAL4ple.PF/StimUAS.cAa

      (Pathak et al., 2017)

      Stimko is partially rescued by Scer\GAL4ple.TH-A/StimUAS.cAa

      (Pathak et al., 2017)
      Comments

      Scer\GAL4nSyb.PS-driven expression of StimScer\UAS.cAa does not rescue small larval body size or the delay in larval development characteristic for Stimko/Stimko but it does partially rescue their lethality - as some adults manage to eclose. Similarly, the lethality but not the developmental delay is partially rescued by Scer\GAL4ple.PF- or Scer\GAL4ple.TH-A-driven expression and the lethality is almost completely rescued when StimScer\UAS.cAa is driven by Scer\GAL4Act5C.PI.

      Co-expression of StimScer\UAS.cAa fully restores the developmental delay characteristic for flies expressing StimsgRNA.dual.snRNA:U6:96Ab together with Spyo\Cas9Scer\UAS.T:nls-Ude under the control of Scer\GAL4elav-C155 (to induce tissue-specific gene knock-out) but their increased lethality is only partially rescued.

      (Pathak et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      STIM1 CRISPR KO
      (Nelson et al., 2019)
      STIMKO
      (Mitra et al., 2024, Kasturacharya et al., 2023)
      STIMko
      (Pathak et al., 2017)
      Stimko
      Name Synonyms
      Secondary FlyBase IDs
        References (4)