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General Information
Symbol
Zzzz\vhhGFP4lexAop.morphotrap.ext.mCherry
Species
a. artificial
Name
FlyBase ID
FBal0326384
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

lexAop regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that consists of the GFP-binding VHH(GFP4) nanobody, the Tag:M(mCd8a) transmembrane domain and the mCherry fluorescent tag. The VHH(GFP4) nanobody is extracellular, allowing it to trap any GFP-tagged extracellular protein, while the mCherry tag is located in the cytosol. (Generated by P1cre-mediated excision of the UAS regulatory sequences present in Zzzz\vhhGFP4lexAop.UAS.morphotrap.ext.mCherry).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The wings of dppd8/dppd12 mutants do not develop at all, only rudimentary stubs are formed, this can be rescued by expression of Ecol\lexALG.dpp>dpplexAop.cHa.EGFP : the wings of these adults display venation defects (extra vein material at the anterior crossvein, loss of distal part of L4) and the have slightly elongated shape but their sizes are comparable to that of control wings. The wing size of these rescued flies can in turn be reduced again by hindering the spreading of the dpp morphogen: completely blocking it by co-expressing Zzzz\vhhGFP4Ecol\lexAop.morphotrap.ext.T:Disc\RFP-mCherry at the source (i.e. within the dpp expression domain) blocks the wing blade development completely.

Blocking dpp spreading at its source however does not interfere with the uniform proliferation pattern in larval imaginal discs as the density of mitotic cells (pH3-positive) is comparable between the rescue and morphotrap discs. Clonal growth rates are also not generally affected (assessed by utilizing the Raeppli tissue-labelling tool), but in the morphotrap wing discs low numbers of very small clones (1-3) are found next to the A/P boundary and these are not observed in the control discs.

In wing discs where dpp spreading is blocked at the source, the growth of the lateral part of the wing compartments shows similar width increase and similar growth rate as in the control discs with dpp spreading unhindered. However, the proliferation of the medial cell population (marked by low brk) is abolished, so the overall size of wing disc is visibly decreased compared to controls.

Similarly, hindering the dpp spreading at its (anterior) source reduces the size of the posterior wing pouch by more than 60%.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Zzzz\vhhGFP4Ecol\lexAop.morphotrap.ext.T:Disc\RFP-mCherry
Zzzz\vhhGFP4lexAop.morphotrap.ext.mCherry
Name Synonyms
Secondary FlyBase IDs
    References (1)