Inhibition (using Ctet\tetX^TNT-LC.UAS or Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R75G12 and Hsap\RELA^AD.R19G02 in adults significantly reduces takeoff rates in response to looming stimuli and almost completely abolishes short-mode takeoffs, compared to controls; in the case of inhibition using Hsap\KCNJ2^{IVS.10xUAS.EGFP}), the giant fiber neuron has significantly reduced excitatory responses to looming and reduced depolarisation in response to slow angular velocity stimuli, compared to controls, with no effect on tonic hyperpolarisation.

Inhibition (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R70G12 and Hsap\RELA^AD.R19G02 in adults results in a reduction in the ability to maintain a light stimulus at an arbitrary azimuthal position during flight, compared to controls, but the ability to fixate on light stimuli frontally is maintained.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 595 nm (amber) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R75G12 and Hsap\RELA^AD.R19G02 in adults results in a large and rapid depolarization of the giant fiber cell, which does not occur in controls.

Inhibition (using Ctet\TeTxLC^TNT.UAS, but not Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R75G12 and Hsap\RELA^AD.R19G02 in adults significantly reduces jump rate in response to looming stimuli, compared to controls.