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General Information
Symbol
Hsap\RELAAD.R19G02
Species
H. sapiens
Name
FlyBase ID
FBal0326410
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R19G02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
EB-IDFP DSB-PB 1 glomerulus neuron

Comment: when combined with P{R70G12-GAL4.DBD} (combination referred to as 'SS00096')

EB-IDFP VSB-PB 1 glomerulus neuron

Comment: when combined with P{R70G12-GAL4.DBD} (combination referred to as 'SS00096')

lobula complex columnar neuron LPLC2

Comment: when combined with P{R75G12-GAL4.DBD} (combination referred to as 'OL0048B')

when combined with P{R12E04-GAL4.DBD} (combination referred to as 'OL0047B ')

Additional Information
Statement
Reference

In a split-GAL4 system, Hsap\RELAAD.R19G02 drives expression in lobula complex columnar neuron LPLC2 when combined with Scer\GAL4DBD.R12E04.

In a split-GAL4 system, Hsap\RELAAD.R19G02 drives expression in lobula complex columnar neuron LPLC2 when combined with Scer\GAL4DBD.R75G12.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\tetXTNT-LC.UAS or Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults significantly reduces takeoff rates in response to looming stimuli and almost completely abolishes short-mode takeoffs, compared to controls; in the case of inhibition using Hsap\KCNJ2IVS.10xUAS.EGFP), the giant fiber neuron has significantly reduced excitatory responses to looming and reduced depolarisation in response to slow angular velocity stimuli, compared to controls, with no effect on tonic hyperpolarisation.

Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R70G12 and Hsap\RELAAD.R19G02 in adults results in a reduction in the ability to maintain a light stimulus at an arbitrary azimuthal position during flight, compared to controls, but the ability to fixate on light stimuli frontally is maintained.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 595 nm (amber) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults results in a large and rapid depolarization of the giant fiber cell, which does not occur in controls.

Inhibition (using Ctet\TeTxLCTNT.UAS, but not Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R75G12 and Hsap\RELAAD.R19G02 in adults significantly reduces jump rate in response to looming stimuli, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\RELAAD.R19G02.T:Zzzz\Zip+
Hsap\RELAAD.R19G02
Name Synonyms
Secondary FlyBase IDs
    References (8)