FB2025_01 , released February 20, 2025
Allele: Hsap\RELAAD.R35D04
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General Information
Symbol
Hsap\RELAAD.R35D04
Species
H. sapiens
Name
FlyBase ID
FBal0326415
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{R35D04-p65.AD}
PBac{R35D04-p65.AD}
Also Known As
R35D04-p65.AD, R35D04-p65ADZp
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wu et al., 2016)
Progenitor genotype
Carried in construct
P{R35D04-p65.AD}
PBac{R35D04-p65.AD}
Cytology
Description

The R35D04 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Wu et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
GMR35D04
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      lobula columnar neuron LC10a

      Comment: when combined with P{R22D06-GAL4.DBD} (combination referred to as 'OL0019B')

      when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

      when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

      when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

      when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

      when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

      when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

      when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

      (Ribeiro et al., 2018, Wu et al., 2016)
      lobula columnar neuron LC10b | subset

      Comment: when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS2')

      when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

      (Ribeiro et al., 2018, Wu et al., 2016)
      lobula columnar neuron LC10a | subset

      Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

      (Wu et al., 2016)
      lobula columnar neuron LC10b

      Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

      when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

      when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

      when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

      when combined with P{VT043656-GAL4.DBD} (combination referred to as 'SS02664')

      when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

      (Wu et al., 2016)
      lobula columnar neuron LC10c

      Comment: when combined with P{R22A07-GAL4.DBD} (combination referred to as 'SS00940')

      when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

      when combined with P{R82D11-GAL4.DBD} (combination referred to as 'OL0021B')

      when combined with P{VT040747-GAL4.DBD} (combination referred to as 'SS02663')

      when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

      (Wu et al., 2016)
      lobula columnar neuron LC12

      Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

      when combined with P{R65B05-GAL4.DBD} (combination referred to as 'OL0007B')

      (Klapoetke et al., 2022, Özel et al., 2021, von Reyn et al., 2017, Wu et al., 2016)
      adult ventral nerve cord | restricted

      Comment: when combined with P{R55F01-GAL4.DBD} (combination referred to as 'OL0008B')

      when combined with P{VT058944-GAL4.DBD} (combination referred to as 'SS02665')

      (Wu et al., 2016)
      lobula columnar neuron LC10d

      Comment: when combined with P{R71E06-GAL4.DBD} (combination referred to as 'SS00938')

      when combined with P{R80E07-GAL4.DBD} (combination referred to as 'OL0020B')

      when combined with P{R91B01-GAL4.DBD} (combination referred to as 'OL0022B')

      when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

      (Wu et al., 2016)
      lobula columnar neuron LC10c | subset

      Comment: when combined with P{VT045997-GAL4.DBD} (combination referred to as 'SS02666')

      (Wu et al., 2016)
      medulla dorsal rim area-posterior lateral protocerebrum neuron

      Comment: when combined with P{R65B05-GAL4.DBD} (combination referred to as 'OL0007B')

      (Kind et al., 2021)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04 reduces the ability of males to orient towards and remain close to a female, compared to controls; they also lose the bias seen in controls to extend the wing closest to, rather than furthest from, the female; following of females, wing extensions and copulation are significantly reduced, compared to controls; courtship of decapitated (immobile) females is unaffected, compared to controls; this inhibition does not affect the ability of adult males to track a rotating grating or a bar, but they are less able to track small fly-sized objects, with no effect on the overall distribution of turning and walking speeds and no effect on movement in the absence of motion stimuli, compared to controls.

      Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.R91B01 and Hsap\RELAAD.R35D04, Scer\GAL4DBD.R22D06 and Hsap\RELAAD.R35D04, or Scer\GAL4DBD.R80E07 and Hsap\RELAAD.R35D04, in adult males does not affect their ability to orient towards and remain close to a female, compared to controls; in the case of Scer\GAL4DBD.R91B01 and Hsap\RELAAD.R35D04, but not Scer\GAL4DBD.R22D06 and Hsap\RELAAD.R35D04, or Scer\GAL4DBD.R80E07 and Hsap\RELAAD.R35D04, wing extensions are significantly reduced, compared to controls, with no effect on following of females.

      Feminization (using traUAS.cFa) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.VT040012, or Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04, in adult males does not affect tracking or courtship of females, compared to controls.

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT043656 and Hsap\RELAAD.R35D04 in adult males results in significantly increased wing extensions, compared to controls, with no increase in turning.

      (Ribeiro et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (15)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Split-GAL4 transgene, constructed as described in FBrf0212052.

      (Wu et al., 2016)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.R35D04.T:Zzzz\Zip+
      Hsap\RELAAD.R35D04
      Name Synonyms
      Secondary FlyBase IDs
        References (17)