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General Information
Symbol
Dmel\Lrp4dalek
Species
D. melanogaster
Name
FlyBase ID
FBal0326703
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

The CRISPR-mediated deletion of the entire coding region of Lrp4 is estimated to extend from 2 bp upstream of the Lrp4 start codon to 34 bp downstream of the stop codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of the entire coding region (as well as the Mi{ET1}Lrp4MB03015 insertion between exons 5 and 6 present in the progenitor).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Lrp4dalek mutants are viable with a slightly reduced body size and show reduction of synapse number (assessed by counting active zone marker puncta) in VA1v olfactory receptor neuron (ORN) axon terminals in females and in ORN axon terminals projecting to the VA1d, DA1, DL4 and DM6 glomeruli as well as in the excitatory neurons projecting to the lateral horn, while no such decrease is observed in the Gad1-positive inhibitory neurons projecting to the DA1 glomerulus or in the inhibitory neurons projecting to the lateral horn. The neurite volume is not affected in any of the neurons except for a slight decrease in the inhibitory lateral horn projecting neurons. On the ultrastructural level, the mutant ORN terminals have slightly increased perimeter and show significant decrease in the T-bar number the majority of which display various morphological defects (including lacking tops, detached, misshapen and frequent multiple T-bars).

No synaptic morphological defects are observed in the VA1v ORNs in Lrp4dalek/+ heterozygous adults.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Neither Lrp4dalek/+ nor Srpk79DP10036/+ single heterozygous adults display any defects in the synapse morphology in the VA1v olfactory receptor neurons but in Lrp4dalek/+;Srpk79DP10036/+ double heterozygotes the synapse number (assessed by counting active zone marker puncta) is significantly decreased compared to controls.

The reduced active zone number in the VA1v olfactory receptor neurons observed in Lrp4dalek homozygous mutants is restored by expression of Srpk79DP10036 under the control of Scer\GAL4peb-GAL4 in the mutant background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (2)