FB2025_01 , released February 20, 2025
Allele: Dmel\KhcUASp.mTagBFP2
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General Information
Symbol
Dmel\KhcUASp.mTagBFP2
Species
D. melanogaster
Name
FlyBase ID
FBal0326707
Feature type
allele
Associated gene
Dmel\Khc
Associated Insertion(s)
Carried in Construct
P{UASp-Khc.mTagBFP2}
Also Known As
UASp-KHC-BFP
Key Links
Transgenic product class
wild_type
(Winding et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Winding et al., 2016)
Mutagen
in vitro construct
(Winding et al., 2016)
Progenitor genotype
Carried in construct
P{UASp-Khc.mTagBFP2}
Cytology
Description

UASp regulatory sequences drive expression of full-length Khc, tagged with mTagBFP2.

(Winding et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Khc
Tagged with
mTagBFP2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      KhcUASp.mTagBFP2/Scer\GAL4ppk.PG rescues Khc27/KhcE177K

      (Kelliher et al., 2018)

      Scer\GAL4VP16.nanos.UTR/KhcUASp.mTagBFP2 rescues KhcKO.mutA

      (Lu et al., 2016)
      Partially rescues

      KhcUASp.mTagBFP2/Scer\GAL4elav.PLu partially rescues KhcKO.mutA

      (Winding et al., 2016)

      KhcUASp.mTagBFP2/Scer\GAL4elav.PLu partially rescues Khc27

      (Winding et al., 2016)
      Comments

      The reduced velocity of ooplasmic streaming characteristic for KhcKO.mutA mutant oocytes is fully rescued by expression of KhcScer\UAS.P\T.T:Equa\eqFP578-TagBFP2 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background.

      (Lu et al., 2016)

      The lethality of Khc27 mutant larvae is strongly delayed by Scer\GAL4elav.PLu-driven expression of KhcScer\UAS.P\T.T:Equa\eqFP578-TagBFP2, but all the Khc27 mutants die before pupariation regardless of the expression the transgene.

      (Winding et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      KhcScer\UAS.P\T.T:Equa\eqFP578-TagBFP2
      KhcUASp.TagBFP2
      KhcUASp.mTagBFP2
      Name Synonyms
      Secondary FlyBase IDs
        References (3)