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General Information
Symbol
Hsap\RELAAD.R13F02
Species
H. sapiens
Name
FlyBase ID
FBal0326965
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The R13F02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
alpha/beta Kenyon cell

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

gamma Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

lobe system of adult mushroom body

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

alpha/beta core Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

alpha/beta posterior Kenyon cell

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R85D07-GAL4.DBD} (combination referred to as 'MB371B')

alpha/beta surface Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

gamma Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

alpha'/beta' Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

alpha/beta Kenyon cell | intense

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

gamma dorsal Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

gamma main Kenyon cell | faint

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

alpha'/beta' Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

alpha/beta core Kenyon cell

Comment: when combined with P{R58F02-GAL4.DBD} (combination referred to as 'MB594B')

gamma dorsal Kenyon cell | faint

Comment: when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

gamma main Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

gamma dorsal Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

gamma main Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

alpha'/beta' anterior-posterior Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

alpha'/beta' middle Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

alpha'/beta' anterior-posterior Kenyon cell | faint

Comment: when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

alpha/beta posterior Kenyon cell | intense

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

alpha'/beta' anterior-posterior Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

alpha'/beta' middle Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

Additional Information
Statement
Reference

In a split-GAL4 system, Hsap\RELAAD.R13F02 drives expression in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha'/beta' anterior-posterior Kenyon cells, alpha'/beta' middle Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with Scer\GAL4DBD.R52H09, in the gamma dorsal Kenyon cells, gamma main Kenyon cells, alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with Scer\GAL4DBD.R21B06, in the the gamma dorsal Kenyon cells and gamma main Kenyon cells when combined with Hsap\RELAAD.R13F02, in the alpha/beta posterior Kenyon cells, alpha/beta surface Kenyon cells and alpha/beta core Kenyon cells when combined with Scer\GAL4DBD.R44E04, in the gamma main Kenyon cells and, stochastically, in the gamma dorsal Kenyon cells when combined with Scer\GAL4DBD.R89B01, in the alpha/beta posterior Kenyon cells when combined with Scer\GAL4DBD.R85D07, and in the alpha'/beta' anterior-posterior Kenyon cells and alpha'/beta' middle Kenyon cells when combined with Scer\GAL4DBD.R34A03 or Scer\GAL4DBD.R41C07.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02 in adults does not affect food intake or climbing rate, compared to controls.

Inhibition (using shits1.UAS.Tag:MYC at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02 in adults during retrieval, but not training or consolidation, significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; inhibition in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed, but not starved, flies, compared to controls.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02 in adults during training, consolidation or retrieval significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; thermogenetic activation in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed or starved flies, compared to controls.

Adult males with inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4DBD.R44E04 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02, or Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02, have significantly weaker suppression of courtship behavior following training with mated females, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R44E04 and Hsap\RELAAD.R13F02 in adult females results in a short depolarization of MBON-alpha1 (mushroom body output neuron 7).

Optogenetic stimulation (using Npha\HRe.UAS.cBa.YFP with 593 nm (yellow) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R41C07 and Hsap\RELAAD.R13F02, or the split-GAL4 pair of Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02, significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls.

Blocking the output (using shits.UAS.cUaat 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R41C07 and Hsap\RELAAD.R13F02 does not affect visual short term memory following training with pain stimulus in adult flies, compared to controls.

Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4DBD.R52H09 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R21B06 and Hsap\RELAAD.R13F02, or Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02 significantly impairs visual (aversive color discrimination conditioning) memory performance, compared to controls.

Blocking synaptic transmission (using shits1.IVS.p10.20xScer\UAS at 33[o]C) from neurons targeted by the split-GAL4 combination of Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R44E04, Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R89B01, Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R85D07, Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R34A03 or Hsap\RELAAD.R13F02 and Scer\GAL4DBD.R41C07 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

Inhibition (using shi1.UAS at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R44E04 and Hsap\RELAAD.R13F02 in adults during, or immediately after, training, but not immediately before testing, significantly impairs long-term appetitive olfactory memory, compared to controls; inhibition does not affect short-term memory.

Thermogenetic activation (using TrpA1UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R41C07 and Hsap\RELAAD.R13F02 in adult males or females significantly decreases the amount of sleep, compared to controls, and results in extra rebound sleep after activation; activation of neurons targeted by Scer\GAL4DBD.R34A03 and Hsap\RELAAD.R13F02 in adult males, but not females, significantly decreases the amount of sleep, compared to controls; activation of neurons targeted by Scer\GAL4DBD.R52H09 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R26E07 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R35B12 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R89B01 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R44E04 and Hsap\RELAAD.R13F02, Scer\GAL4DBD.R85D07 and Hsap\RELAAD.R13F02, or Scer\GAL4DBD.R18F09 and Hsap\RELAAD.R13F02, in adult males or females does not significantly affect the amount of sleep, compared to controls; activation of neurons targeted by any of these combinations does not significantly affect locomotor activity of awake adult males, compared to controls.

Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R52H09 and Hsap\RELAAD.R13F02 in adults significantly reduces aversive and appetitive 1-min olfactory memory, compared to controls; inhibition during training or testing alone significantly reduces aversive 30-min visual memory, compared to controls inhibition does not significantly affect responses to sugar or electric shock, compared to controls.

Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R45H04 and Hsap\RELAAD.R13F02 in adults significantly reduces aversive and appetitive 1-min olfactory memory, compared to controls; inhibition during testing alone, but not training, significantly reduces aversive 30-min visual memory, compared to controls inhibition does not significantly affect responses to sugar or electric shock, compared to controls.

Inhibition (using shi1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R44E04 and Hsap\RELAAD.R13F02 in adults does not significantly affect aversive or appetitive 1-min olfactory memory, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (9)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

"Split GAL4" system transgene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Hsap\RELAAD.13F02.T:Zzzz\Zip+
Hsap\RELAAD.13F02.T:Zzzz\ZipEERRL
Hsap\RELAAD.R13F02.T:Zzzz\Zip+
Hsap\RELAAD.R13F02
Name Synonyms
Secondary FlyBase IDs
  • FBal0304494
  • FBal0290920
References (25)