FlyBase Allele Report: Hsap\RELA[AD.R13F02]

General Information

Symbol

Hsap\RELA^AD.R13F02

Species

H. sapiens

Name

FlyBase ID

FBal0326965

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R13F02-p65.AD}

PBac{R13F02-p65.AD}

Also Known As

R13F02-p65ADZp, 13F02-p65ADZp, R13F02-p65.AD

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014, Bräcker et al., 2013)

Progenitor genotype

Carried in construct

P{R13F02-p65.AD}

PBac{R13F02-p65.AD}

Cytology

Description

The R13F02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR13F02

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

alpha/beta Kenyon cell

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

(Pankova and Borst, 2017, Ichinose et al., 2015, Vogt et al., 2014)

gamma Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Yamazaki et al., 2018, Aso et al., 2014, Vogt et al., 2014)

lobe system of adult mushroom body

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2014)

alpha/beta core Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016, Aso et al., 2014)

alpha/beta posterior Kenyon cell

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R85D07-GAL4.DBD} (combination referred to as 'MB371C')

when combined with P{R85D07-GAL4.DBD} (combination referred to as 'MB371B')

(Li et al., 2020, Vogt et al., 2016, Aso et al., 2014)

alpha/beta surface Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016, Aso et al., 2014)

gamma Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

(Aso et al., 2014)

alpha'/beta' Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

(Aso et al., 2014)

alpha/beta Kenyon cell | intense

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Aso et al., 2014)

gamma dorsal Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016, Aso et al., 2014)

gamma main Kenyon cell | faint

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

(Aso et al., 2014)

alpha'/beta' Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Aso et al., 2014)

alpha/beta core Kenyon cell

Comment: when combined with P{R58F02-GAL4.DBD} (combination referred to as 'MB594B')

(Aso et al., 2014)

gamma dorsal Kenyon cell | faint

Comment: when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

(Vogt et al., 2016, Aso et al., 2014)

gamma main Kenyon cell

Comment: when combined with P{R45H04-GAL4.DBD} (combination referred to as 'MB009B')

when combined with P{R89B01-GAL4.DBD} (combination referred to as 'MB131B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016, Aso et al., 2014)

gamma dorsal Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

(Vogt et al., 2016)

gamma main Kenyon cell | intense

Comment: when combined with P{R21B06-GAL4.DBD} (combination referred to as 'MB364B')

(Vogt et al., 2016)

alpha'/beta' anterior-posterior Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

(Vogt et al., 2016)

alpha'/beta' middle Kenyon cell

Comment: when combined with P{R34A03-GAL4.DBD} (combination referred to as 'MB005B')

when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

(Vogt et al., 2016)

alpha'/beta' anterior-posterior Kenyon cell | faint

Comment: when combined with P{R41C07-GAL4.DBD} (combination referred to as 'MB370B')

(Vogt et al., 2016)

alpha/beta posterior Kenyon cell | intense

Comment: when combined with P{R44E04-GAL4.DBD} (combination referred to as 'MB008B')

when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016)

alpha'/beta' anterior-posterior Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016)

alpha'/beta' middle Kenyon cell | intense

Comment: when combined with P{R52H09-GAL4.DBD} (combination referred to as 'MB010B')

(Vogt et al., 2016)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R34A03

(Zhao et al., 2018)

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R44E04

(Zhao et al., 2018)

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R45H04

(Zhao et al., 2018)

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R89B01

(Zhao et al., 2018)

abnormal memory | adult stage, with Dop1R1^HMC02344, Scer\GAL4^DBD.R44E04

(Ichinose et al., 2015)

abnormal memory | adult stage, with Inx5^KK103391, Scer\GAL4^DBD.R41C07

(Liu et al., 2016)

abnormal memory | adult stage, with Inx6^GD3692, Scer\GAL4^DBD.R41C07

(Liu et al., 2016)

abnormal memory | adult stage | :heat sensitive | nutrition conditional, with Scer\GAL4^DBD.R89B01, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal memory | adult stage | conditional, with Npha\HR^{eNpHR2.0.UAS.cBa.YFP}, Scer\GAL4^DBD.R41C07

(Liu et al., 2016)

abnormal memory | adult stage | conditional, with Npha\HR^{eNpHR2.0.UAS.cBa.YFP}, Scer\GAL4^DBD.R89B01

(Liu et al., 2016)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R21B06, shi^{ts1.IVS.p10.20xUAS}

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R44E04, shi^1.UAS

(Ichinose et al., 2015)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R45H04, shi^1.UAS

(Vogt et al., 2014)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R45H04, shi^{ts1.IVS.p10.20xUAS}

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R52H09, shi^1.UAS

(Vogt et al., 2014)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R52H09, shi^{ts1.IVS.p10.20xUAS}

(Vogt et al., 2016)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R89B01, shi^{ts1.UAS.Tag:MYC}

(Yamazaki et al., 2018)

abnormal memory | adult stage | heat sensitive, with Scer\GAL4^DBD.R89B01, TrpA1^UAS.(B).cKa

(Yamazaki et al., 2018)

abnormal neuroanatomy | adult stage, with Hsap\SNCA^A53T.UAS.cPa, Scer\GAL4^DBD.R44E04

(Praschberger et al., 2023)

abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R44E04

(Takemura et al., 2017)

abnormal neurophysiology | adult stage | conditional, with Cnoc\ChR1^{Chrimson.IVS.Syn21.10xUAS.tdTomato}, Scer\GAL4^DBD.R44E04

(Takemura et al., 2017)

abnormal sleep | adult stage | heat sensitive, with Scer\GAL4^DBD.R41C07, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

abnormal sleep | adult stage | male limited | heat sensitive, with Scer\GAL4^DBD.R34A03, TrpA1^UAS.(B).cKa

(Sitaraman et al., 2015)

chemical resistant | :nutrition conditional | heat sensitive, with Scer\GAL4^DBD.R52H09, shi^1.UAS

(Bräcker et al., 2013)

increased sleep | adult stage, with Lar^HMS02186, Scer\GAL4^DBD.R45H04

(Draper et al., 2024)

increased sleep | adult stage, with Scer\GAL4^DBD.R45H04, nkt^{UAS.t.I.Tag:MYC}

(Draper et al., 2024)

Phenotype Manifest In

mushroom body output neuron 7 | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R44E04

(Takemura et al., 2017)

mushroom body output neuron 7 | conditional, with Cnoc\ChR1^{Chrimson.IVS.Syn21.10xUAS.tdTomato}, Scer\GAL4^DBD.R44E04

(Takemura et al., 2017)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02 in adults does not affect food intake or climbing rate, compared to controls.

(Al-Anzi and Zinn, 2018)

Inhibition (using shi^{ts1.UAS.Tag:MYC} at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 in adults during retrieval, but not training or consolidation, significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; inhibition in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed, but not starved, flies, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02 in adults during training, consolidation or retrieval significantly reduces 2 h aversive, or appetitive, olfactory memory, compared to controls; thermogenetic activation in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory in fed or starved flies, compared to controls.

(Yamazaki et al., 2018)

Adult males with inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pairs of Scer\GAL4^DBD.R44E04 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R34A03 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02, or Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, have significantly weaker suppression of courtship behavior following training with mated females, compared to controls.

(Zhao et al., 2018)

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 627 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R44E04 and Hsap\RELA^AD.R13F02 in adult females results in a short depolarization of MBON-alpha1 (mushroom body output neuron 7).

(Takemura et al., 2017)

Optogenetic stimulation (using Npha\HR^{e.UAS.cBa.YFP} with 593 nm (yellow) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02, or the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, significantly impairs visual short term memory following training with pain stimulus in adult flies, compared to controls.

Blocking the output (using shi^ts.UAS.cUaat 30[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02 does not affect visual short term memory following training with pain stimulus in adult flies, compared to controls.

(Liu et al., 2016)

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Scer\GAL4^DBD.R52H09 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R21B06 and Hsap\RELA^AD.R13F02, or Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02 significantly impairs visual (aversive color discrimination conditioning) memory performance, compared to controls.

Blocking synaptic transmission (using shi^{ts1.IVS.p10.20xScer\UAS} at 33[o]C) from neurons targeted by the split-GAL4 combination of Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R44E04, Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R89B01, Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R85D07, Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R34A03 or Hsap\RELA^AD.R13F02 and Scer\GAL4^DBD.R41C07 does not significantly impair visual (aversive color discrimination conditioning) memory performance, compared to controls.

(Vogt et al., 2016)

Inhibition (using shi^1.UAS at 32[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R44E04 and Hsap\RELA^AD.R13F02 in adults during, or immediately after, training, but not immediately before testing, significantly impairs long-term appetitive olfactory memory, compared to controls; inhibition does not affect short-term memory.

(Ichinose et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02 in adult males or females significantly decreases the amount of sleep, compared to controls, and results in extra rebound sleep after activation; activation of neurons targeted by Scer\GAL4^DBD.R34A03 and Hsap\RELA^AD.R13F02 in adult males, but not females, significantly decreases the amount of sleep, compared to controls; activation of neurons targeted by Scer\GAL4^DBD.R52H09 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R26E07 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R35B12 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R44E04 and Hsap\RELA^AD.R13F02, Scer\GAL4^DBD.R85D07 and Hsap\RELA^AD.R13F02, or Scer\GAL4^DBD.R18F09 and Hsap\RELA^AD.R13F02, in adult males or females does not significantly affect the amount of sleep, compared to controls; activation of neurons targeted by any of these combinations does not significantly affect locomotor activity of awake adult males, compared to controls.

(Sitaraman et al., 2015)

Inhibition (using shi^1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R52H09 and Hsap\RELA^AD.R13F02 in adults significantly reduces aversive and appetitive 1-min olfactory memory, compared to controls; inhibition during training or testing alone significantly reduces aversive 30-min visual memory, compared to controls inhibition does not significantly affect responses to sugar or electric shock, compared to controls.

Inhibition (using shi^1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R45H04 and Hsap\RELA^AD.R13F02 in adults significantly reduces aversive and appetitive 1-min olfactory memory, compared to controls; inhibition during testing alone, but not training, significantly reduces aversive 30-min visual memory, compared to controls inhibition does not significantly affect responses to sugar or electric shock, compared to controls.

Inhibition (using shi^1.UAS at 33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R44E04 and Hsap\RELA^AD.R13F02 in adults does not significantly affect aversive or appetitive 1-min olfactory memory, compared to controls.

(Vogt et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions