FlyBase Allele Report: Dmel\E(spl)mγ-HLH[UAS.cLb]

General Information

Symbol

Dmel\E(spl)mγ-HLH^UAS.cLb

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct b of Liu

FlyBase ID

FBal0327027

Feature type

allele

Associated gene

Dmel\E(spl)mγ-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)mγ-HLH.Lb}

Key Links

Transgenic product class

cDNA

(Liu et al., 2017)

wild_type

(Liu et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Liu et al., 2017)

wild_type

(Liu et al., 2017)

Mutagen

in vitro construct

(Liu et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-E(spl)mγ-HLH.Lb}

Cytology

Description

UAS regulatory sequences drive expression of E(spl)mγ-HLH cDNA.

(Liu et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)mγ-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

increased cell number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Phenotype Manifest In

larval brain | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

neuroblast | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Detailed Description

Statement

Reference

The expression of E(spl)mγ-HLH^Scer\UAS.cLb under the control of Scer\GAL4^insc-Mz1407 leads to a severe increase in the number of neuroblasts per third instar larval brain lobe, as compared to controls.

(Liu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has increased cell number | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has increased cell number | third instar larval stage phenotype, suppressible | partially by Su(Tpl)^HMS00277, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Su(Tpl)^HMS00277, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

NOT suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has increased cell number | third instar larval stage phenotype, non-suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Suppressor of

Statement

Reference

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of increased cell number | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has larval brain | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has larval brain | third instar larval stage phenotype, suppressible | partially by Su(Tpl)^HMS00277, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has neuroblast | third instar larval stage phenotype, suppressible by Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has neuroblast | third instar larval stage phenotype, suppressible | partially by Su(Tpl)^HMS00277, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

NOT suppressed by

Statement

Reference

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has neuroblast | third instar larval stage phenotype, non-suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb, Scer\GAL4^insc-Mz1407 has larval brain | third instar larval stage phenotype, non-suppressible by N^GD144, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Suppressor of

Statement

Reference

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of neuroblast | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

E(spl)mγ-HLH^UAS.cLb/Cdk9^D199N.UAS.SR, Scer\GAL4^insc-Mz1407 is a suppressor of larval brain | third instar larval stage phenotype of N^{UAS.Tag:V5,Tag:polyHis}, Scer\GAL4^insc-Mz1407

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of neuroblasts in third instar larval brain lobes resulting from the expression of E(spl)mγ-HLH^Scer\UAS.cLb under the control of Scer\GAL4^insc-Mz1407 is fully suppressed by the co-expression of Cdk9^{D199N.Scer\UAS.SR}, strongly suppressed by the co-expression of Su(Tpl)^HMS00277, but not suppressed by the co-expression of N^GD144 (and Dicer-2 transgene, for efficient RNAi).

The combined co-expression of E(spl)mγ-HLH^Scer\UAS.cLb and Cdk9^{D199N.Scer\UAS.SR} partially suppresses the increased number of neuroblasts resulting from the expression of N^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^insc-Mz1407 in third instar larval brains.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E(spl)mγ-HLH^Scer\UAS.cLb

E(spl)mγ-HLH^UAS.cLb

Name Synonyms

Saccharomyces cerevisiae UAS construct b of Liu

Secondary FlyBase IDs

References (1)

Report Sections

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