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General Information
Symbol
Hsap\FUSUAS.cIa
Species
H. sapiens
Name
Saccharomyces cerevisiae UAS construct a of Ishiguro
FlyBase ID
FBal0327174
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Hsap\FUS.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS leads to a mild rough eye phenotype, as compared to controls.

The progressive eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS is accelerated at 28[o]C when it is clearly visible already at day 1 post-eclosion, whereas at 25[o]C the phenotype is not manifested until day 6. The eye degeneration phenotype is even stronger when two copies of the Hsap\FUSScer\UAS.cIa transgene are used and the flies cultured at 28[o]C - under these extreme conditions the ommatidial lattice is completely obliterated.

A significant increase in apoptosis (assessed by [*]Casp3 immunolabelling) is observed in third larval instar eye discs expressing Hsap\FUSScer\UAS.cIa under Scer\GAL4GMR.PS.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

Hsap\FUSUAS.cIa, Scer\GAL4GMR.PS has visible | adult stage phenotype, non-enhanceable by Atg8ad4/Atg8a[+]

Suppressed by
NOT suppressed by
Suppressor of
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

Hsap\FUSUAS.cIa, Scer\GAL4GMR.PS has eye phenotype, non-enhanceable by Atg8ad4/Atg8a[+]

Suppressed by
NOT suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The additional expression of Hsap\FUSScer\UAS.cIa leads to partial decreases in the severity of the eye defects either resulting from the Scer\GAL4GMR.PS-driven expression of Zzzz\UGGAAW.Scer\UAS alone or the combined expression of Zzzz\UGGAAW.Scer\UAS and TBPHGD6943 (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities).

The co-expression of Hsap\FUSScer\UAS.cIa rescues the lethality resulting from the co-expression of Zzzz\UGGAAS.Scer\UAS and TBPHGD6943 under the control of Scer\GAL4GMR.PS; the rescued adults exhibit significantly less severe eye phenotypes, as compared to individuals only expressing Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS.

The eye defects observed upon the expression of Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS are partially suppressed by the co-expression of Zzzz\UGGAA22.Scer\UAS.

The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS is completely rescued by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS but not BacA\p35Scer\UAS.cHa, Diap1Scer\UAS.cHa) or Hsap\LAMP1Scer\UAS.T:Arus\HRP. The complete rescue of the eye defects by lncRNA:HsrωdsRNA.Sym.Scer\UAS expression is however prevented by combination with a single copy of Lamp1MI15062 as a vast majority of Lamp1MI15062 heterozygotes expressing both Hsap\FUSScer\UAS.cIa and lncRNA:HsrωdsRNA.Sym.Scer\UAS under Scer\GAL4GMR.PS display areas of degeneration in the eyes.

The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) under Scer\GAL4GMR.PS is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy).

The extreme eye degeneration phenotype (complete loss of the ommatidial lattice), observed when two copies of Hsap\FUSScer\UAS.cIa are expressed and the flies kept at 28[o]C, is rather weakly suppressed by expression of one copy of lncRNA:HsrωdsRNA.Sym.Scer\UAS and more strongly when two copies are used but even then the rescue remains incomplete under these conditions.

The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy).

The increase in the number of apoptotic ([*]Casp3-positive) cells in third instar larval eye discs observed upon Scer\GAL4GMR.PS-expression of Hsap\FUSScer\UAS.cIa is partially suppressed by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\FUSScer\UAS.cIa
Hsap\FUSUAS.cIa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Ishiguro
Secondary FlyBase IDs
    References (3)