The additional expression of Hsap\FUSScer\UAS.cIa leads to partial decreases in the severity of the eye defects either resulting from the Scer\GAL4GMR.PS-driven expression of Zzzz\UGGAAW.Scer\UAS alone or the combined expression of Zzzz\UGGAAW.Scer\UAS and TBPHGD6943 (i.e. reduced size, loss of pigmentation, defective morphology and ommatidial abnormalities).
The co-expression of Hsap\FUSScer\UAS.cIa rescues the lethality resulting from the co-expression of Zzzz\UGGAAS.Scer\UAS and TBPHGD6943 under the control of Scer\GAL4GMR.PS; the rescued adults exhibit significantly less severe eye phenotypes, as compared to individuals only expressing Zzzz\UGGAAS.Scer\UAS under the control of Scer\GAL4GMR.PS.
The eye degeneration and the associated rough eye appearance, ommatidial fusion and pigmentation loss characteristic for adult flies expressing Hsap\FUSScer\UAS.cIa under the control of Scer\GAL4GMR.PS is completely rescued by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS but not BacA\p35Scer\UAS.cHa, Diap1Scer\UAS.cHa) or Hsap\LAMP1Scer\UAS.T:Arus\HRP. The complete rescue of the eye defects by lncRNA:HsrωdsRNA.Sym.Scer\UAS expression is however prevented by combination with a single copy of Lamp1MI15062 as a vast majority of Lamp1MI15062 heterozygotes expressing both Hsap\FUSScer\UAS.cIa and lncRNA:HsrωdsRNA.Sym.Scer\UAS under Scer\GAL4GMR.PS display areas of degeneration in the eyes.
The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) under Scer\GAL4GMR.PS is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy).
The extreme eye degeneration phenotype (complete loss of the ommatidial lattice), observed when two copies of Hsap\FUSScer\UAS.cIa are expressed and the flies kept at 28[o]C, is rather weakly suppressed by expression of one copy of lncRNA:HsrωdsRNA.Sym.Scer\UAS and more strongly when two copies are used but even then the rescue remains incomplete under these conditions.
The eye degeneration induced by Hsap\FUSScer\UAS.cIa expression (one copy) is not exacerbated by combination with Atg8ad4/+ and the eye defects in the Scer\GAL4GMR.PS/Atg8ad4;Hsap\FUSScer\UAS.cIa/+ flies can still be rescued by expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS (one copy).
The increase in the number of apoptotic ([*]Casp3-positive) cells in third instar larval eye discs observed upon Scer\GAL4GMR.PS-expression of Hsap\FUSScer\UAS.cIa is partially suppressed by co-expression of lncRNA:HsrωdsRNA.Sym.Scer\UAS.