FB2025_01 , released February 20, 2025
Allele: Dmel\gclG2A.UASp.Tag:FLAG,Tag:HA
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General Information
Symbol
Dmel\gclG2A.UASp.Tag:FLAG,Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0327517
Feature type
allele
Associated gene
Dmel\gcl
Associated Insertion(s)
Carried in Construct
P{UASp-gcl.G2A.FLAG.HA}
Key Links
Transgenic product class
missense_variant
(Pae et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Pae et al., 2017)
Mutagen
in vitro construct
(Pae et al., 2017)
Progenitor genotype
Carried in construct
P{UASp-gcl.G2A.FLAG.HA}
Cytology
Description

UASp regulatory sequences drive expression of gcl with a mutation in the N-terminal glycine (G2A) required for myristoylation-mediated membrane targeting, tagged with 3xTag:FLAG and 3xTag:HA.

(Pae et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
gcl
Tagged with
Tag:FLAG
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      gclG2A.UASp.Tag:FLAG,Tag:HA/Scer\GAL4VP16.nanos.UTR fails to rescue gclrev390

      (Pae et al., 2017)
      Comments

      The expression of gclG2A.Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the severe decrease in primordial germ cells in the early embryo (i.e. mitotic cycles 12/13) of gclrev390 homozygotes.

      (Pae et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      gclG2A.Scer\UAS.P\T.T:Zzzz\FLAG,T:Ivir\HA1
      gclG2A.UASp.Tag:FLAG,Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)