FlyBase Allele Report: Dmel\axed[2094]

General Information

Symbol

Dmel\axed²⁰⁹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0327536

Feature type

allele

Associated gene

Dmel\axed

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Neukomm et al., 2017)

Progenitor genotype

Cytology

Description

May be a regulatory mutation, as a molecular lesion within the axed ORF could not be identified.

(Neukomm et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing | adult stage

(Neukomm et al., 2017)

lethal | recessive

(Neukomm et al., 2017)

Phenotype Manifest In

axon | adult stage

(Neukomm et al., 2017)

Detailed Description

Statement

Reference

Flies carrying axed²⁰⁹⁴ mutant somatic clones show defective injury-induced axon degeneration: severed axons (either in sensory neurons in the wing or in cholinergic olfactory receptor neurons) remain intact for a full week after an axotomy (or even 50 days in the latter case), instead of being cleared away. After axotomy of mechanosensory neurons in the Johnston's organ, the persisting severed axons can still elicit grooming behavior upon optogenetic stimulation of the neurons.

(Neukomm et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Nmnat^Δ4790-1, axed²⁰⁹⁴ has abnormal wound healing | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut1-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Suppressor of

Statement

Reference

axed²⁰⁹⁴ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

axed²⁰⁹⁴ is a non-suppressor of visible | adult stage phenotype of hid^GMR.PG

(Neukomm et al., 2017)

Other

Statement

Reference

Nmnat^Δ4790-1, axed²⁰⁹⁴ has abnormal wound healing | adult stage phenotype

(Neukomm et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Nmnat^Δ4790-1, axed²⁰⁹⁴ has sensory neuron | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut1-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Nmnat^Δ4790-1, axed²⁰⁹⁴ has axon | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut1-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Nmnat^Δ4790-1, axed²⁰⁹⁴ has neuronal cell body | somatic clone | adult stage phenotype, non-suppressible by Scer\GAL4^VGlut1-OK371/Sarm^{ΔARM.UAS.Tag:MYC}

(Neukomm et al., 2017)

Suppressor of

Statement

Reference

axed²⁰⁹⁴ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of neuronal cell body | somatic clone | adult stage phenotype of Nmnat^Δ4790-1

(Neukomm et al., 2017)

axed²⁰⁹⁴/Sarm⁸⁹⁶ is a suppressor of axon | somatic clone | adult stage phenotype of Nmnat^GD8082

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of axon | somatic clone | adult stage phenotype of Sarm^{ΔARM.UAS.Tag:MYC}, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

axed²⁰⁹⁴ is a suppressor of sensory neuron | somatic clone | adult stage phenotype of Nmnat^GD8082, Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

NOT Suppressor of

Statement

Reference

axed²⁰⁹⁴ is a non-suppressor of eye phenotype of hid^GMR.PG

(Neukomm et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

MARCM clones in sensory neurons in the adult wing expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^VGlut-OK371 undergo spontaneous cell body and axon degeneration which can be suppressed by combination with axed²⁰⁹⁴.

The axon and cell body degeneration in sensory neuron clones in the adult wing mutant for Nmnat^Δ4790-1 or expressing Nmnat^GD8082 under the control of Scer\GAL4^VGlut-OK371 can be fully rescued by combination with axed²⁰⁹⁴ or by axed²⁰⁹⁴ and Ect4⁸⁹⁶ together.

The Nmnat^Δ4790-1,axed²⁰⁹⁴ double mutant clones display defective injury-induced axon degeneration (severed axons remain intact following an axotomy, instead of being cleared away) which cannot be overcome by expressing Ect4^{ΔARM.Scer\UAS.T:Hsap\MYC} under the Scer\GAL4^VGlut-OK371 driver in the mutant clones.

The small eye phenotype resulting from the cell death induced by expression of hid^GMR.PG is not suppressed by combination with axed²⁰⁹⁴.

(Neukomm et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

axed^MI13270

(Neukomm et al., 2017)

Rescued by

axed²⁰⁹⁴ is rescued by Scer\GAL4^VGlut1-OK371/axed^UAS.long

(Neukomm et al., 2017)

Partially rescued by

axed²⁰⁹⁴ is partially rescued by Scer\GAL4^VGlut1-OK371/axed^ΔBTB.UAS

(Neukomm et al., 2017)

axed²⁰⁹⁴ is partially rescued by axed^ΔBACK.UAS/Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Not rescued by

axed²⁰⁹⁴ is not rescued by axed^ΔCterm.UAS/Scer\GAL4^VGlut1-OK371

(Neukomm et al., 2017)

Comments

The defective injury-induced axon degeneration observed in flies carrying axed²⁰⁹⁴ mutant neural clones (severed axons in sensory neurons in the adult wing remain intact following an axotomy, instead of being cleared away) can be rescued by clonal Scer\GAL4^VGlut-OK371-driven expression of any of the following: axed^{Scer\UAS.long}, axed^{ΔBTB.Scer\UAS}, axed^{ΔBACK.Scer\UAS} or axed^{ΔCterm.Scer\UAS}.

Any of the following: axed⁰⁰¹¹, axed^CrisprA, axed^CrisprB, axed^CrisprC, axed^Ex07, axed^Ex65 or axed^Ex96 fails to complement the lethality of axed²⁰⁹⁴ mutants, while Ect4⁸⁹⁶ fails to do so.

(Neukomm et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

3L.2094

(Neukomm et al., 2017)

axed²⁰⁹⁴

(Llobet Rosell et al., 2022, Neukomm et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (2)

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