FlyBase Allele Report: Scer\GAL4[kn-MI15480-TG4.2]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Scer\GAL4^{kn-MI15480-TG4.2}

General Information

Symbol

Scer\GAL4^{kn-MI15480-TG4.2}

Species

S. cerevisiae

Name

FlyBase ID

FBal0327926

Feature type

allele

Associated gene

Associated Insertion(s)

Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}

Carried in Construct

Also Known As

Kn-Gal4

Genomic Maps

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Gene Disruption Project members, 2016-)

Progenitor genotype

Associated Insertion(s)

Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}

Cytology

Description

The RMCE cassette of the Mi{MIC}kn^MI15480 insertion has been replaced with a Trojan-Gal4 cassette. The insertion is in a coding intron of kn and is predicted to gene trap the gene. (Note that if this gene has multiple transcripts, only those where the insertion is in a coding intron are predicted to be trapped).

(Gene Disruption Project members, 2016-)

Allele components

Component

Use(s)

Inserted element

Mi{Trojan-GAL4.2}

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,778,860..14,778,860 [-]

(Hirschhäuser et al., 2023, Özel et al., 2021, Xie et al., 2021, Allen et al., 2020, Davis et al., 2020, Konstantinides et al., 2018, Lee et al., 2018, Chao et al., 2017, Gene Disruption Project members, 2016-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

anterior-posterior compartment boundary of the wing disc | restricted

(Chao et al., 2017)

wing hinge primordium | restricted

(Chao et al., 2017)

larval central nervous system | restricted

(Chao et al., 2017)

transmedullary Y neuron TmY14

(Davis et al., 2020, Konstantinides et al., 2018)

transmedullary Y neuron TmY5a

(Davis et al., 2020)

lobula columnar neuron LC17

(Özel et al., 2021)

lineage 23 secondary neuron | subset

(Allen et al., 2020)

hemilineage 9B secondary neuron | subset

(Allen et al., 2020)

hemilineage 8B secondary neuron | subset

(Allen et al., 2020)

Additional Information

Statement

Reference

ScerGAL4^{kn-MI15480-TG4.2} recapitulates endogenous kn expression in the wing disc and in the CNS.

(Chao et al., 2017)

Marker for

Reflects expression of

(Özel et al., 2021, Allen et al., 2020, Konstantinides et al., 2018, Bloomington Drosophila Stock Center, 2017, Chao et al., 2017)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\EBF3^{WT.UAS.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2} is a suppressor of lethal - all die during embryonic stage phenotype of Df(2R)BSC429/kn^{MI15480-TG4.2}

(Chao et al., 2017)

NOT Suppressor of

Statement

Reference

Hsap\EBF3^{Arg163Gln.UAS.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2} is a non-suppressor of lethal - all die during embryonic stage phenotype of Df(2R)BSC429/kn^{MI15480-TG4.2}

(Chao et al., 2017)

Scer\GAL4^{kn-MI15480-TG4.2}/Hsap\EBF3^{Arg163Leu.UAS.Tag:HA} is a non-suppressor of lethal - all die during embryonic stage phenotype of Df(2R)BSC429/kn^{MI15480-TG4.2}

(Chao et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

kn^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2} rescues Df(2R)BSC429/kn^{MI15480-TG4.2}

(Lee et al., 2018)

kn^{UAS.ORF.GW.Tag:HA}/Scer\GAL4^{kn-MI15480-TG4.2} rescues Df(2R)BSC429/kn^{MI15480-TG4.2}

(Chao et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

67516

y¹ w^*; Mi{Trojan-GAL4.2}kn^{MI15480-TG4.2}/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{kn-MI15480-TG4.2}

kn^GAL4

(Allen et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections