FlyBase Allele Report: Hsap\RELA[AD.R21F05]

General Information

Symbol

Hsap\RELA^AD.R21F05

Species

H. sapiens

Name

FlyBase ID

FBal0328516

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R21F05-p65.AD}

Also Known As

R21F05

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R21F05-p65.AD}

Cytology

Description

The GMR21F05 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR21F05

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval excitatory interneuron eIN-3

Comment: when combined with P{R58E05-GAL4.DBD} (combination referred to as 'SS01379')

(Zwart et al., 2016)

adult stage

descending neuron of the anterior ventral brain DNb02

Comment: when combined with P{R93B10-GAL4.DBD} (combination referred to as 'SS02396')

(Namiki et al., 2022, Namiki et al., 2018)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

uncoordinated | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R58E05

(Zwart et al., 2016)

Phenotype Manifest In

muscle cell of lateral transverse muscle 2 | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R58E05

(Zwart et al., 2016)

Detailed Description

Statement

Reference

Acute, high intensity optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R58E05 and Hsap\RELA^AD.R21F05 in larvae induces contraction of transverse muscle LT2, but not longitudinal muscle LO1, compared to baseline; chronic, low-level stimulation causes transverse muscle LT2 to contract earlier than normal in the locomotor cycle, reducing the phase offset between LT2 and LO1 contractions, compared to baseline.

(Zwart et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions