FlyBase Allele Report: Hsap\RELA[AD.R52B10]

General Information

Symbol

Hsap\RELA^AD.R52B10

Species

H. sapiens

Name

FlyBase ID

FBal0329003

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R52B10-p65.AD}

PBac{R52B10-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R52B10-p65.AD}

PBac{R52B10-p65.AD}

Cytology

Description

The GMR52B10 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR52B10

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval abdominal 3 A09e neuron

Comment: when combined with P{R70C11-GAL4.DBD} (combination referred to as 'SS00878')

(Jovanic et al., 2019)

larval abdominal 4 A09e neuron

Comment: when combined with P{R70C11-GAL4.DBD} (combination referred to as 'SS00878')

(Jovanic et al., 2019)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | larval stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R70C11

(Jovanic et al., 2019)

decreased rate of movement | larval stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL4^DBD.R70C11

(Jovanic et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\TeTxLC[TNT.UAS]) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R70C11 and Hsap\RELA^AD.R52B10 in larvae significantly reduces navigation away from higher wind speeds, compared to controls; movement speed in the absense of wind stimuli is also significantly reduced, compared to controls; turn rate and size of turns are similar to controls.

(Jovanic et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions