FB2025_01 , released February 20, 2025
Allele: Hsap\RELAAD.VT040232
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General Information
Symbol
Hsap\RELAAD.VT040232
Species
H. sapiens
Name
FlyBase ID
FBal0330569
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{VT040232-p65.AD}
Also Known As
VT040232.p65ADZp
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Rubin Laboratory at Janelia Farm, 2017)
Progenitor genotype
Carried in construct
P{VT040232-p65.AD}
Cytology
Description

The VT040232 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)
Allele components
Component
Use(s)
Regulatory region(s)
VT040232
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      wing basalar muscle b2 motor neuron

      Comment: when combined with P{VT023830-GAL4.DBD} (combination referred to as 'b2-SG')

      (O'Sullivan et al., 2018)
      dorsal tp motor neuron

      Comment: when combined with P{VT029310-GAL4.DBD} (combination referred to as 'tp1,tp2-SG')

      (O'Sullivan et al., 2018)
      ventral tp motor neuron

      Comment: when combined with P{VT029310-GAL4.DBD} (combination referred to as 'tp1,tp2-SG')

      when combined with P{VT042475-GAL4.DBD} (combination referred to as 'tp2-SG2')

      (O'Sullivan et al., 2018)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT042475 and Hsap\RELAAD.VT040232, or the split-GAL4 pair of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, in adult males results in a significant reduction in the number of males that sing courtship song to females, compared to controls; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, there also significant alterations in some parameters of pulse song, but not sine song; most individuals have altered wing posture (drooping), compared to controls, but males with drooping wings still extend a wing toward females; take off and forced flight ability are also significantly impaired, compared to controls; in the case of Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, but not Scer\GAL4DBD.VT042475 and Hsap\RELAAD.VT040232, there is a significant reduction in copulation success, compared to controls.

      Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (yellow) light) of neurons targeted by the split-GAL4 pair of the split-GAL4 pair of Scer\GAL4DBD.VT042475 and Hsap\RELAAD.VT040232, or Scer\GAL4DBD.VT029310 and Hsap\RELAAD.VT040232, in adult males results in wing lifting.

      Silencing (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT023830 and Hsap\RELAAD.VT040232 in adult males does not affect courtship song, wing extension toward females, or copulation success, compared to controls; take off and forced flight ability are significantly impaired, compared to controls.

      (O'Sullivan et al., 2018)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (3)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.VT040232.T:Zzzz\Zip+
      Hsap\RELAAD.VT040232
      Name Synonyms
      Secondary FlyBase IDs
        References (5)