FlyBase Allele Report: Scer\GAL4[DBD.R42F06]

General Information

Symbol

Scer\GAL4^DBD.R42F06

Species

S. cerevisiae

Name

FlyBase ID

FBal0331867

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R42F06-GAL4.DBD}

Also Known As

R42F06-DBD, R42F06-ZpGdbd, R42F06DBD

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R42F06-GAL4.DBD}

Cytology

Description

The GMR42F06 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR42F06

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

T4 neuron

Comment: when combined with P{R59E08-p65.AD}

when combined with P{R59E08-p65.AD} (combination referred to as 'SS00324')

when combined with P{R59E08-p65.AD} (combination referred to as 'T4/T5')

when combined with P{R59E08-p65.AD} (combination referred to as 'T4/T5-SplitGal4')

when combined with P{VT015785-p65.AD} (combination referred to as 'SS02344 (combination genotype confirmed with the authors)))

when combined with P{VT055812-p65.AD} (combination referred to as 'SS21452 (combination genotype confirmed with the authors)))

(Tanaka and Clark, 2022, Davis et al., 2020, Drews et al., 2020, Hörmann et al., 2020, Keleş et al., 2020, Strother et al., 2017, Bahl et al., 2015)

T5 neuron

Comment: when combined with P{R59E08-p65.AD}

when combined with P{R59E08-p65.AD} (combination referred to as 'SS00324')

when combined with P{R59E08-p65.AD} (combination referred to as 'T4/T5')

when combined with P{R59E08-p65.AD} (combination referred to as 'T4/T5-SplitGal4')

when combined with P{VT055812-p65.AD} (combination referred to as 'SS21452 (combination genotype confirmed with the authors)))

(Tanaka and Clark, 2022, Davis et al., 2020, Drews et al., 2020, Hörmann et al., 2020, Keleş et al., 2020, Strother et al., 2017, Bahl et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior, with Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R59E08

(Ribeiro et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\tetX^TNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R42F06 and Hsap\RELA^AD.R59E08 reduces the ability of adult males to orient towards and remain close to a female, compared to controls; they also lose the bias seen in controls to extend the wing closest to, rather than furthest from, the female; following of females and wing extensions, but not copulation, are significantly reduced, compared to controls.

(Ribeiro et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions