FlyBase Allele Report: Scer\GAL4[DBD.R58F03]

General Information

Symbol

Scer\GAL4^DBD.R58F03

Species

S. cerevisiae

Name

FlyBase ID

FBal0332145

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R58F03-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R58F03-GAL4.DBD}

Cytology

Description

The GMR58F03 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR58F03

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval excitatory interneuron eIN-1

Comment: when combined with P{VT059793-p65.AD} (combination referred to as 'SS01970')

(Zwart et al., 2016)

adult stage

adult lateral horn PV4c3 neuron

Comment: when combined with P{R11E08-p65.AD} (combination referred to as 'LH1397')

(Dolan et al., 2019)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

uncoordinated | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.VT059793

(Zwart et al., 2016)

Phenotype Manifest In

muscle cell of lateral transverse muscle 2 | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.VT059793

(Zwart et al., 2016)

Detailed Description

Statement

Reference

Acute, high intensity optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R58F03 and Hsap\RELA^AD.VT059793 in larvae induces contraction of transverse muscle LT2, but not longitudinal muscle LO1, compared to baseline; chronic, low-level stimulation causes transverse muscle LT2 to contract earlier than normal in the locomotor cycle, reducing the phase offset between LT2 and LO1 contractions, compared to baseline.

(Zwart et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions