FlyBase Allele Report: Scer\GAL4[DBD.R60E02]

General Information

Symbol

Scer\GAL4^DBD.R60E02

Species

S. cerevisiae

Name

FlyBase ID

FBal0332177

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{R60E02-GAL4.DBD}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R60E02-GAL4.DBD}

Cytology

Description

The GMR60E02 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR60E02

Encoded product / tool

GAL4(DBD)::Zip-

split driver - DNA-binding fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

Comment: when combined with P{R39A11-p65.AD}

(Hampel et al., 2015)

adult ventral nerve cord | restricted

Comment: when combined with P{R39A11-p65.AD}

(Hampel et al., 2015)

zone C/E Johnston organ neuron

Comment: when combined with P{R39A11-p65.AD}

when combined with P{R52F12-p65.AD}

(Hampel et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Hsap\RELA^AD.R52F12

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Hsap\RELA^AD.R39A11, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Hsap\RELA^AD.R52F12, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the following split-GAL4 pairs results in a significant increase of antennal grooming behavior, compared to controls: Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12, Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R39A11.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12 induces antennal grooming, which persists for tens of seconds beyond red light cessation.

(Hampel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R52F12, Scer\GAL4^DBD.R60E02 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Inhibition of synaptic release (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA1^{13xlexAop.JFRC26} at 30-31[o]C) of Ecol\lexA^GMR27H08 neurons.

(Hampel et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

69533

w¹¹¹⁸; P{R60E02-GAL4.DBD}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{DBD.R60E02.T:Zzzz\Zip-}

Scer\GAL4^DBD.R60E02

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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