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General Information
Symbol
Scer\GAL4DBD.VT002081
Species
S. cerevisiae
Name
FlyBase ID
FBal0332774
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The VT002081 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain).

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\TeTxLCTNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT002081 and Hsap\RELAAD.R23E07 in third instar larvae significantly reduces the ability of larvae to navigate to an appetitive odor, compared to controls; there is no difference in run speed, compared to controls; turn rate is significantly reduced when larvae are moving down, but not up, an odor gradient, and not in the absence of odor, compared to controls; the probability of turning towards, rather an away from, an appetitive odor source is not affected, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 625 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT002081 and Hsap\RELAAD.R23E07 in third instar larvae significantly increases stopping and head-casting behavior, compared to controls; the stopping phenotype is not affected by the odor environment, whereas the head casting phenotype is more likely and more vigorous when larvae are moving down, rather than up, an odor gradient.

Unilateral single-cell optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus and the low activity flippase Scer\FLP1G5.R57C10.Tag:PEST(mOdc), activated with 625 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.VT002081 and Hsap\RELAAD.R23E07 in third instar larvae is sufficient to induce stopping and head-casting behaviors, relative to baseline.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.VT002081.T:Zzzz\Zip-
Scer\GAL4DBD.VT002081
Name Synonyms
Secondary FlyBase IDs
    References (3)