Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT029310 in adult males results in a complete loss of courtship song in response to females, and altered wing posture (drooping) in all males, compared to controls; males with drooping wings still extend wings toward females as often as controls, but large wing extension angles are observed significantly less often and bilateral extension occurs significantly more often than in controls; copulation success is significantly reduced; take off and forced flight ability are also significantly impaired, compared to controls.

Silencing (using Ctet\TeTxLC^TNT.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969, or the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT040232, in adult males results in a significant reduction in the number of males that sing courtship song to females, in the case of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969, this is accompanied by significant alterations in some parameters of pulse song, but not sine song; few (Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969) or most (Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT040232) individuals have altered wing posture (drooping), compared to controls; there is no overall effect on the pattern of wing extension toward females and males with drooping wings still perform this behavior; copulation success is not significantly affected, but take off and forced flight ability are significantly impaired, compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 590 nm (yellow) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT045969, or the split-GAL4 pair of Scer\GAL4^DBD.VT042475 and Hsap\RELA^AD.VT040232, in adult males results in wing lifting.