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General Information
Symbol
Dmel\Kdm4BKO
Species
D. melanogaster
Name
FlyBase ID
FBal0335406
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

The Kdm4B ORF was replaced with a knock-in cassette containing a Red Fluorescent Protein (RFP) transgene. Replaced region determined from reported primer sequences.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The endogenous Kdm4B open reading frame has been replaced by a loxP cassette which contains both Disc\RFP3xP3.PC and the kanamycin resistance gene.

Insertion components
TI{TI}Kdm4BKO
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Kdm4BKO mutant males are less viable relative to controls (only about 64% of the expected number survives to adulthood), but otherwise the Kdm4BKO flies are fertile and do not display significant developmental timing defects. Kdm4BKO suppresses the variegated phenotype of the w or Sb locus in the In(1)wm4 or T(2;3)SbV background, respectively.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
NOT Enhancer of
NOT Suppressor of
Phenotype Manifest In
Enhancer of
Statement
Reference

Kdm4B[+]/Kdm4BKO is an enhancer of wing vein phenotype of Scer\GAL4C-765, smo5A.UAS.Tag:FLAG

NOT Enhancer of
Statement
Reference

Kdm4B[+]/Kdm4BKO is a non-enhancer of eye phenotype of Scer\GAL4ey.200.Exel, TAF1BdsRNA.UAS

NOT Suppressor of
Statement
Reference

Kdm4B[+]/Kdm4BKO is a non-suppressor of eye phenotype of Scer\GAL4ey.200.Exel, TAF1BdsRNA.UAS

Additional Comments
Genetic Interactions
Statement
Reference

Only about 20% of Kdm4AKO,Kdm4BKO double mutants survive to adulthood, while Kdm4BKO;JHDM2KO double mutants are viable and fertile.

Combination with a single copy of Kdm4BKO does not modify the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1BdsRNA.Scer\UAS under the control of Scer\GAL4ey.200.Exel nor the massive eye overgrowth in flies expressing ykiS168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1 with Scer\GAL4GMR.PF but it exacerbates the disrupted wing vein phenotype of flies expressing smo5A.Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4C-765.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)