UAS regulatory sequences (derived from pWALIUM20) drive expression of a short hairpin RNA (shRNA) that targets moon.
female sterile, with Scer\GAL4VP16.nanos.UTR, Scer\GAL4VP16.otu, Scer\GAL4nanos.PG
The expression of moonshRNA.Scer\UAS under the combined control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 leads to female sterility, as all embryos fail to hatch.