FlyBase Allele Report: Dmel\cnk[63F]

General Information

Symbol

Dmel\cnk^63F

Species

D. melanogaster

Name

FlyBase ID

FBal0336631

Feature type

allele

Associated gene

Dmel\cnk

Associated Insertion(s)

Carried in Construct

Genomic Maps

GBrowse JBrowse

Help me choose

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wolfstetter et al., 2017)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

2R:17,419,419..17,419,419 [-]

Nucleotide change:

G17419419A

Amino acid change:

W17term | cnk-PA; W17term | cnk-PB

Reported amino acid change:

W17term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Wolfstetter et al., 2017)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: W17term.

(Wolfstetter et al., 2017)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal

(Wolfstetter et al., 2017)

lethal (with cnk^ΔY2H)

(Wolfstetter et al., 2017)

visible | adult stage, with cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

visible | adult stage (with cnk^CC9-110B)

(Wolfstetter et al., 2017)

visible | adult stage (with cnk^ΔY2H)

(Wolfstetter et al., 2017)

Phenotype Manifest In

embryonic/larval heart visceral muscle | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

embryonic/larval midgut longitudinal visceral muscle | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

embryonic Malpighian tubule | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

embryonic pericardial cell | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

embryonic somatic muscle | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

eye, with cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

eye (with cnk^CC9-110B)

(Wolfstetter et al., 2017)

eye (with cnk^ΔY2H)

(Wolfstetter et al., 2017)

muscle attachment site | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

muscle founder cell | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

trachea | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

Detailed Description

Statement

Reference

cnk^63F/Df(2R)BSC161 embryos derived from mothers bearing cnk^63F mutant germline clones and thus lacking both maternal and zygotic cnk show a complete loss of muscle founder cells at late embryonic stages. The embryos also display mild defects in the formation of ectodermal and endodermal derivatives of the alimentary tract (hindgut and Malpighian tubules are shortened) as well as severe heart and dorsal muscle formation defects, absence of eve-positive pericardial cells, impaired longitudinal visceral muscle migration, abnormal muscle attachment sites and impaired tracheal development.

cnk^63F;cnk^fTRG1248 rescue flies survive to adulthood but display rough eye phenotype.

(Wolfstetter et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

cnk[+]/cnk^63F is a suppressor | partially of size defective | pupal stage phenotype of Scer\GAL4^elav-C155, jeb^UAS.cVa

(Wolfstetter et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced pupal size characteristic for animals expressing jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155 is partially suppressed by combination with a single copy of cnk^63F.

(Wolfstetter et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

jeb^k05644

(Wolfstetter et al., 2017)

Alk¹

(Wolfstetter et al., 2017)

Alk¹⁰

(Wolfstetter et al., 2017)