UASt regulates expression of an dsRNA construct targeting Glos for RNAi-mediated degradation.
lethal, with Scer\GAL4c179
lethal, with Scer\GAL4how-24B
lethal, with Scer\GAL4insc-Mz1407
lethal - all die before end of P-stage, with Scer\GAL4da.G32
lethal - all die before end of P-stage, with Scer\GAL4αTub84B.PL
locomotor behavior defective | adult stage | progressive, with Scer\GAL4elav-C155
locomotor behavior defective | adult stage | progressive, with Scer\GAL4Mef2.PR
partially lethal, with Scer\GAL4insc-Mz1407
short lived, with Scer\GAL4elav-C155
Expression of GlosGD17786 under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.
Expression of GlosGD17786, together with UAS-Dicer2 to enhance RNAi efficiency, is lethal at both 25[o]C or 29[o]C if driven by Scer\GAL4αTub84B.PL, Scer\GAL4da.G32, Scer\GAL4how-24B or Scer\GAL4c179 and at 25[o]C only if driven by Scer\GAL4insc-Mz1407 (but only partially lethal at 29[o]C). Adult viability is not significantly reduced if GlosGD17786 is controlled by Scer\GAL4RapGAP1-OK6, Scer\GAL4elav-C155, Scer\GAL4G7 or Scer\GAL4Mef2.PR. Scer\GAL4αTub84B.PL-, Scer\GAL4da.G32- or Scer\GAL4c179-driven expression is lethal at both temperatures (and at just 29[o]C with Scer\GAL4how-24B) even without the UAS-Dicer2.
The climbing (especially at late adulthood) as well as the flight ability of flies expressing GlosGD17786 (with UAS-Dicer2) under the control of either Scer\GAL4Mef2.PR or Scer\GAL4elav-C155 is strongly reduced and the adult survival rate is also significantly decreased with the Scer\GAL4elav-C155 driver.
Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has lethal - all die before end of P-stage phenotype
Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has locomotor behavior defective | third instar larval stage phenotype
Gem3ΔN.UAS, GlosGD17786, Scer\GAL4Mef2.PR has size defective | pupal stage phenotype
Gem3ΔN.UAS, GlosGD17786, Scer\GAL4elav-C155 has partially lethal - majority die phenotype
Expression of Gem3ΔN.Scer\UAS together with GlosGD17786 are adult lethal and leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4Mef2.PR driver and to reduced viability with Scer\GAL4elav-C155.