FlyBase Allele Report: Ecol\lexA[GMR94B10]

General Information

Symbol

Ecol\lexA^GMR94B10

Species

E. coli

Name

FlyBase ID

FBal0337172

Feature type

allele

Associated gene

Ecol\lexA

Associated Insertion(s)

Carried in Construct

P{GMR94B10-lexA}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Yoshino et al., 2017)

Progenitor genotype

Carried in construct

P{GMR94B10-lexA}

Cytology

Description

The R94B10 enhancer is fused upstream of the Drosophila synthetic core promoter (DSCP), followed by a lexA::p65 driver that has been optimised for Drosophila codon usage. An Hsp70 transcriptional terminator is present.

(Yoshino et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR94B10

Encoded product / tool

lexA::p65

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | larval stage | conditional, with Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.lexAop.Citrine}

(Yoshino et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Optogenetic activation (using Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.lexAop.Citrine} with 617 nm (red) light) of Ecol\lexA^GMR94B10 cells in larvae results in an induction of nociceptive rolling behavior in the absence of nociceptive stimuli, unlike controls.

(Yoshino et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.lexAop.Citrine}, Ecol\lexA^GMR94B10 has abnormal pain response | larval stage | conditional phenotype, non-enhanceable by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^GMR69F06

(Yoshino et al., 2017)

Suppressed by

Statement

Reference

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.lexAop.Citrine}, Ecol\lexA^GMR94B10 has abnormal pain response | larval stage | conditional phenotype, suppressible | partially by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^B-H1.B4.5

(Yoshino et al., 2017)

NOT suppressed by

Statement

Reference

Crei\ChR1::Vcar\ChR1::Vcar\ChR2^{ReaChR.lexAop.Citrine}, Ecol\lexA^GMR94B10 has abnormal pain response | larval stage | conditional phenotype, non-suppressible by Ctet\tetX^TNT-LC.UAS/Scer\GAL4^GMR69F06

(Yoshino et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ecol\lexA^GMR94B10

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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