Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^NP4820 cells in larvae results in a significant reduction in the time they spend in the vicinity of an appetitive odor, or a dark region, and they navigate less successfully to a preferred temperature range, compared to controls; these larvae have significantly longer runs with significantly reduced turn frequency; when turns occur, larvae orient towards odor stimuli at the same rate as controls, but turning motion is affected, with backwards movement and slower head casts. Restriction of the number of inhibited cells using Scer\GAL80^ChAT.3.3P significantly suppresses navigation phenotypes in odor, light and temperature gradients; restriction using Scer\GAL80^{tsh-md621-GAL80} or Scer\GAL80^Mef2.mb247 does not alter the effects of inhibition on odor gradient navigation.

Inhibition (using Ctet\tetX^{TNT-LC.E86.FRT.STOP.10xUAS}) of Scer\GAL4^NP4820 cells expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^{tsh-md621-lexA} in larvae does not reduce the time they spend in the vicinity of an appetitive odor, and does not significantly affect turn frequency or run length, compared to controls; expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^p65.R57C10.P, instead, significantly decreases turn frequency and increases run length in the presence of an appetitive odor, but does not reduce the overall time spent in the odor vicinity, compared to controls.

Acute optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 625 nm (red) light) of Scer\GAL4^NP4820 cells in larvae evokes reliable head casts, unlike controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at greater than 28[o]C) of Scer\GAL4^NP4820 cells, with activation further restricted using Scer\GAL80^{tsh-md621-GAL80}, in larvae results in a significant increase in angular head speed, compared to controls; this is not the case when Scer\GAL80^ChAT.3.3P is used instead of Scer\GAL80^{tsh-md621-GAL80}.