FB2025_01 , released February 20, 2025
Allele: Dmel\STUB1I
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General Information
Symbol
Dmel\STUB1I
Species
D. melanogaster
Name
FlyBase ID
FBal0337738
Feature type
allele
Associated gene
Dmel\STUB1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Chen et al., 2017)
Progenitor genotype
Cytology
Description

Frameshift mutation: 9 base pairs from positions 14 to 22 of the coding sequence have been deleted and substituted with GTCACGA. This is predicted to result in a truncated protein of 29 amino acid residues.

(Chen et al., 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
delins
2L:10,437,852..10,437,860 [+]
Inserted_sequence:

GTCACGA

Comment:

The deletion of 9 base pairs and insertion of 7 (GTCACGA) leads to a frameshift and early translation terminaion.

(Chen et al., 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Alzheimer's disease 1
      modeled by Hsap\APP695.UAS
      (Zhuang et al., 2020)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      STUB1I mutant adults exhibit reduced climbing ability along with age-progressive mitochondrial defects (decreased thoracic mtDNA content and disorganization of the cristae).

      (Chen et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhancer of
      Statement
      Reference

      STUB1I/STUB1I is a non-enhancer of abnormal locomotor behavior | adult stage phenotype of parkΔ21/park1

      (Chen et al., 2017)
      NOT Suppressor of
      Statement
      Reference

      STUB1I/STUB1I is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1B9

      (Chen et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The reduced climbing ability of park1/parkΔ21 mutants is not exacerbated by combination with STUB1I in homozygous state, but the lifespan of the double mutants is significantly decreased compared to park1/parkΔ21 single mutants. Similarly, the climbing deficit observed in Pink1B9 mutants is also not exacerbated by combination with STUB1I in homozygous state; lifespan of the double mutants is not significantly different from the Pink1B9 single mutants.

      (Chen et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      STUB1I is rescued by STUB1UAS.cCa/Scer\GAL4Mhc.PU

      (Chen et al., 2017)
      Comments

      The reduced climbing ability of STUB1I mutant adults is rescued by Scer\GAL4Mhc.PU-driven expression of STUB1Scer\UAS.cCa.

      (Chen et al., 2017)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CHIP mutant I
      (Chen et al., 2017)
      CHIP1
      (Zhuang et al., 2020)
      STUB1I
      Name Synonyms
      Secondary FlyBase IDs
        References (2)