FB2025_01 , released February 20, 2025
Allele: Dmel\hpo4A-431T.αTub84B.FRT.Tag:FLAG
Open Close
General Information
Symbol
Dmel\hpo4A-431T.αTub84B.FRT.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0337743
Feature type
allele
Associated gene
Dmel\hpo
Associated Insertion(s)
Carried in Construct
P{αTub(FRT.y+)FLAG-hpo.4A-431T}
Key Links
Transgenic product class
missense_variant
(Zheng et al., 2017)
polypeptide_post_translational_processing_variant
(Zheng et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Zheng et al., 2017)
polypeptide_post_translational_processing_variant
(Zheng et al., 2017)
Mutagen
in vitro construct
(Zheng et al., 2017)
Progenitor genotype
Carried in construct
P{αTub(FRT.y+)FLAG-hpo.4A-431T}
Cytology
Description

An FRT cassette containing a transcription stop signal and a y+t* marker separates a αTub84B promoter from a mutated hpo coding region that carries mutations in 4 of the 5 autophosphorylation sites of the linker region (T356A, T374A, T387A, T421A). The 5th autophosphorylation site T431 has not been mutated (this residue is also part of a mats protein binding site in the wild-type protein).

(Zheng et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
αTub84B
Encoded product / tool
hpo
Tagged with
Tag:FLAG
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In animals with muscle-specific hpo4A-431T.αTub84B.FRT.Tag:FLAG clones, the indirect flight muscles show atrophy and muscle fiber compaction after 32h APF.

      (Kaya-Çopur et al., 2021)

      Flies expressing hpo4A-431T.αTub84B.FRT.T:Zzzz\FLAG from a tubulin promoter in the eye through Scer\FLP1ey.PU-mediated excision of an intervening FRT cassette from the transgene leads to pupal lethality.

      (Zheng et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      hippo4A/431T
      (Kaya-Çopur et al., 2021)
      hpo4A-431T.αTub84B.FRT.T:Zzzz\FLAG
      hpo4A-431T.αTub84B.FRT.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)