FB2021_01, released Feb 18, 2021

Allele: Dmel\bratG774D.UAS.RNAi-res.Tag:FLAG.Tag:MYC

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General Information
Symbol
Dmel\bratG774D.UAS.RNAi-res.Tag:FLAG.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0337828
Feature type
allele
Associated gene
Dmel\brat
Associated Insertion(s)
Carried in Construct
P{UAS-brat.G774D.3xFlag6xMyc.RNAi-res}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Reichardt et al., 2018)
in vitro construct - coding region fusion
(Reichardt et al., 2018)
in vitro construct - amino acid replacement
(Reichardt et al., 2018)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-brat.G774D.3xFlag6xMyc.RNAi-res}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UAS regulatory sequences drive expression of a mutated brat open reading frame (ORF) carrying the amino acid replacement G774D. The ORF is tagged at the N-terminal end with both Tag:FLAG and Tag:MYC. The brat coding sequence has also undergone nucleotide substitutions so that it is resistant to RNAi mediated by the P{KK113206} construct.

      (Reichardt et al., 2018)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      brat
      Tagged with
      Tag:FLAG
      Tag:MYC
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4wor.PA, Scer\GAL80ase.PN, bratG774D.UAS.RNAi-res.Tag:FLAG.Tag:MYC rescues bratKK113206

      (Reichardt et al., 2018)
      Comments

      The co-expression of bratG774D.Scer\UAS.RNAi-res.T:Zzzz\FLAG.T:Hsap\MYC rescues the larval brain tumor phenotype observed upon the expression of bratKK113206 under the combined control of Scer\GAL4wor.PA and Scer\GAL80ase.PN.

      (Reichardt et al., 2018)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      bratG774D.Scer\UAS.RNAi-res.T:Zzzz\FLAG.T:Hsap\MYC
      bratG774D.UAS.RNAi-res.Tag:FLAG.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (1)