FlyBase Allele Report: Dmel\trbl[R141E.UAS.Tag:FLAG]

General Information

Symbol

Dmel\trbl^{R141E.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0338077

Feature type

allele

Associated gene

Dmel\trbl

Associated Insertion(s)

Carried in Construct

P{UAS-FLAG-trbl.R141E}

Key Links

Transgenic product class

missense_variant

(Fischer et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Fischer et al., 2017)

Mutagen

in vitro construct

(Fischer et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-FLAG-trbl.R141E}

Cytology

Description

UAS regulatory sequences drive expression of trbl carrying a R141E amino acid replacement. The location of this mutation in trbl is equivalent to the rare Q/R84 SNP found in the human TRIB3 gene (R at this position in the human gene is associated with a predisposition to early onset insulin resistance and early onset Type 2 diabetes). The protein is tagged at the N-terminal end with Tag:FLAG.

(Fischer et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

trbl

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Fischer et al., 2017)

Reverse comparison required, since wild-type fly gene carries Arg at the structurally analogous position.

Phenotypic Data

Phenotypic Class

abnormal cell migration | oogenesis, with Scer\GAL4^slbo.2.6

(Fischer et al., 2017)

decreased cell size | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Fischer et al., 2017)

embryonic/larval fat body | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

fat cell | larval stage, with Scer\GAL4^r4

(Fischer et al., 2017)

larva, with Scer\GAL4^r4

(Fischer et al., 2017)

wing hair, with Scer\GAL4^en-e16E

(Fischer et al., 2017)

Detailed Description

Statement

Reference

Larvae expressing trbl^{R141E.UAS.Tag:FLAG} under the control of Scer\GAL4^r4 show a significant decrease in body weight; their fat body cells are significantly smaller than in controls.

The expression of trbl^{R141E.UAS.Tag:FLAG} under the control of Scer\GAL4^slbo.2.6 induces a severe block in border cell migration.

The expression of trbl^{R141Q.UAS.Tag:FLAG} under the control of Scer\GAL4^en-e16E leads to a significant reduction in wing size:trichome density ratio, despite of no strong differences in wing size, as compared to controls.

(Fischer et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

trbl^{R141E.Scer\UAS.T:Zzzz\FLAG}

trbl^{R141E.UAS.Tag:FLAG}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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