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General Information
Symbol
Dmel\enaUAS.Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0340223
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ena, UAS-ena.His6
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of ena tagged with Tag:polyHis.

UASt regulatory sequences drive expression of a wild-type ena cDNA. The protein is tagged with Tag:polyHis.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Third instar larvae expressing enaScer\UAS.T:Zzzz\His6 under the control of Scer\GAL4RapGAP1-OK6 show normal number of satellite boutons on neuromuscular junctions relative to controls.

Third instar larvae expressing enaScer\UAS.cCa under the control of Scer\GAL4how-24B show a significant decrease in the number of synaptic boutons and in presynaptic arbor expansion at the neuromuscular junction compared to controls. There is a significant reduction in subsynaptic reticulum thickness, area and fold complexity compared to controls, while presynaptic bouton area is normal.

Larvae expressing enaScer\UAS.cCa under the control of Scer\GAL4how-24B show no significant changes in evoked excitatory junctional potential (EJP) amplitude, miniature EJP (mEJP) amplitude, mEJP frequency and quantal content at the neuromuscular junction compared to controls.

Expression of enaScer\UAS.cCa under the control of Scer\GAL4how-24B results in a reduction in the size and complexity of the muscle 6/7 neuromuscular junction. A reduced number of boutons is seen, along with reduced expansion of the presynaptic terminal arbor and fewer axonal branches. The neuromuscular junctions of larvae expressing enaScer\UAS.cCa under the pan-neural Scer\GAL4elav.PLu driver are indistinguishable from controls.

Over expression of enaScer\UAS.cCa in the wing using Scer\GAL4Bx-MS1096 results in tissue overgrowth.

Larvae expressing enaScer\UAS.cCa under the control of Scer\GAL4elav-C155, Scer\GAL4hs.PB or Scer\GAL4Act5C.PI do not show axonal transport phenotypes.

Expression of enaScer\UAS.cCa under the control of Scer\GAL4CY2 or Scer\GAL4mat.αTub67C.T:Hsim\VP16 induces the formation of punctate cytoplasmic F-actin structures associated with clumps of ena protein.

ISNb bypass phenotypes are not produced when enaScer\UAS.cCa is expressed under the control of Scer\GAL4elav.PLu.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Tissue overgrowth resulting from expression of enaScer\UAS.cCa in the wing using Scer\GAL4Bx-MS1096 is not enhanced by co-expression of picoScer\UAS.T:Zzzz\His6,T:Hsap\MYC.

Tissue overgrowth resulting from expression of enaScer\UAS.cCa in the wing using Scer\GAL4Bx-MS1096 is suppressed by co-expressing a copy of picoIR.Scer\UAS.

Expression of enaScer\UAS.cCa under the control of Scer\GAL4elav.PLu suppresses the midline crossing phenotype seen in transheterozygous sli2 robo1 stage 16 embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Symbol Synonym
enaScer\UAS.T:Zzzz\His6.cMa
enaScer\UAS.T:Zzzz\His6
enaScer\UAS.cCa
enaUAS.Tag:polyHis
enbScer\UAS.cCa
enbUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Comer
Secondary FlyBase IDs
  • FBal0241978
  • FBal0086641
References (14)