FlyBase Allele Report: Dmel\Kdm5[140]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\Kdm5¹⁴⁰

General Information

Symbol

Dmel\Kdm5¹⁴⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0341385

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

Allele class

amorphic allele

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele

(Zamurrad et al., 2018)

amorphic allele - molecular evidence

(Drelon et al., 2018)

Mutagen

Delta2-3 transposase

(Drelon et al., 2018)

Progenitor genotype

P{GawB}Kdm5^NP4707

(Drelon et al., 2018)

Cytology

Description

Deletion that removes three of the four coding exons of lid, including the start codon.

(Drelon et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

2L:5,992,105..5,998,014 [-]

Comment:

A 5910bp deletion resulting from the imprecise excision of P{GawB}Kdm5^NP4707 that removes three of the four coding exons of Kdm5.

(Drelon et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Yheskel et al., 2023)

model of syndromic X-linked intellectual disability Claes-Jensen type

CEA

(Belalcazar et al., 2021)

model of syndromic intellectual disability

CEA

(Hatch et al., 2021)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of intellectual disability

is ameliorated by Kdm5^{UASp.C.Tag:HA,TurboID}

(Yheskel et al., 2023)

is ameliorated by Kdm5^{UASp.N.Tag:HA,TurboID}

(Yheskel et al., 2023)

model of syndromic intellectual disability

is exacerbated by pros¹⁷

(Hatch et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

abnormal behavior | adult stage, with Kdm5^JmjC.Tag:HA

(Yheskel et al., 2024)

abnormal behavior | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

abnormal behavior | adult stage, with Kdm5^R873W.Tag:HA

(Yheskel et al., 2024)

abnormal behavior | adult stage, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

abnormal developmental rate

(Drelon et al., 2019)

abnormal developmental rate | larval stage

(Belalcazar et al., 2021)

abnormal developmental rate | larval stage | recessive

(Drelon et al., 2018)

abnormal DNA repair | conditional

(Drelon et al., 2018)

abnormal endomitotic cell cycle | third instar larval stage

(Drelon et al., 2019)

abnormal locomotor behavior | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

abnormal locomotor behavior | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

abnormal neuroanatomy | adult stage, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

abnormal neuroanatomy | adult stage, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

abnormal neuroanatomy | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

abnormal neuroanatomy | adult stage, with Kdm5^R873W.Tag:HA

(Yheskel et al., 2024)

abnormal neuroanatomy | adult stage, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

abnormal neuroanatomy | third instar larval stage

(Belalcazar et al., 2021)

abnormal neuroanatomy | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

abnormal neuroanatomy | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

abnormal neurophysiology | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

(Drelon et al., 2018)

bang sensitive | adult stage, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

bang sensitive | adult stage, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

bang sensitive | adult stage, with Kdm5^JmjC.Tag:HA

(Yheskel et al., 2024)

bang sensitive | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

bang sensitive | adult stage, with Kdm5^R873W.Tag:HA

(Yheskel et al., 2024)

bang sensitive | adult stage, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of long-term memory | adult stage, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of long-term memory | adult stage, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of long-term memory | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of short-term memory | adult stage, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of short-term memory | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

decreased efficacy of short-term memory | adult stage, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

decreased occurrence of cell division | third instar larval stage

(Drelon et al., 2018)

decreased occurrence of cell division | wandering third instar larval stage

(Drelon et al., 2019)

decreased rate of adult locomotory behavior, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

decreased rate of adult locomotory behavior, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

decreased rate of adult locomotory behavior, with Kdm5^JmjC.Tag:HA

(Yheskel et al., 2024)

decreased rate of adult locomotory behavior, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

decreased rate of adult locomotory behavior, with Kdm5^R873W.Tag:HA

(Yheskel et al., 2024)

decreased rate of adult locomotory behavior, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

female semi-sterile, with Kdm5^UASp.cSa, Scer\GAL4^Mai60

(Drelon et al., 2019)

increased cell death | third instar larval stage

(Drelon et al., 2018)

increased cell death | wandering third instar larval stage

(Drelon et al., 2019)

(Drelon et al., 2019, Drelon et al., 2018)

male sterile, with Kdm5^UASp.cSa, Scer\GAL4^Mai60

(Drelon et al., 2019)

partially lethal - majority live, with Kdm5^R873W.Tag:HA

(Belalcazar et al., 2021)

partially lethal - majority live, with Kdm5^Y874C.Tag:HA

(Belalcazar et al., 2021)

semi-fertile, with Kdm5^UASp.cSa, Scer\GAL4^Ubi.PU

(Drelon et al., 2019)

short lived, with Kdm5^UASp.cSa, Scer\GAL4^Mai60

(Drelon et al., 2019)

short lived, with Kdm5^UASp.cSa, Scer\GAL4^spok.1.45

(Drelon et al., 2019)

sterile, with Kdm5^UASp.cSa, Scer\GAL4^spok.1.45

(Drelon et al., 2019)

viable, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

visible | adult stage, with Kdm5^UASp.cSa, Scer\GAL4^32B

(Drelon et al., 2019)

visible | adult stage, with Kdm5^UASp.cSa, Scer\GAL4^Mai60

(Drelon et al., 2019)

visible | adult stage, with Kdm5^UASp.cSa, Scer\GAL4^phtm.PO

(Drelon et al., 2019)

visible | adult stage, with Kdm5^UASp.cSa, Scer\GAL4^spok.1.45

(Drelon et al., 2019)

Phenotype Manifest In

bouton | decreased number | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

bouton | decreased number | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

bouton | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

embryonic/larval neuromuscular junction | third instar larval stage

(Belalcazar et al., 2021)

embryonic/larval neuromuscular junction | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

embryonic/larval neuromuscular junction | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

lobe system of adult mushroom body | adult stage, with Kdm5^A224T.Tag:HA

(Yheskel et al., 2024)

lobe system of adult mushroom body | adult stage, with Kdm5^D234G.Tag:HA

(Yheskel et al., 2024)

lobe system of adult mushroom body | adult stage, with Kdm5^L854F.Tag:HA

(Yheskel et al., 2024)

lobe system of adult mushroom body | adult stage, with Kdm5^R873W.Tag:HA

(Yheskel et al., 2024)

lobe system of adult mushroom body | adult stage, with Kdm5^Y874C.Tag:HA

(Yheskel et al., 2024)

microtubule | third instar larval stage

(Belalcazar et al., 2021)

microtubule | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

prothoracic gland | third instar larval stage

(Drelon et al., 2019)

type Ib terminal bouton | decreased number | third instar larval stage

(Belalcazar et al., 2021)

type Ib terminal bouton | decreased number | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

type Ib terminal bouton | decreased number | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

type Ib terminal bouton | third instar larval stage

(Belalcazar et al., 2021)

type Ib terminal bouton | third instar larval stage, with Kdm5^JmjC.Tag:HA

(Belalcazar et al., 2021)

type Ib terminal bouton | third instar larval stage, with Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

wing, with Kdm5^UASp.cSa, Scer\GAL4^32B

(Drelon et al., 2019)

wing, with Kdm5^UASp.cSa, Scer\GAL4^Mai60

(Drelon et al., 2019)

wing, with Kdm5^UASp.cSa, Scer\GAL4^phtm.PO

(Drelon et al., 2019)

wing, with Kdm5^UASp.cSa, Scer\GAL4^spok.1.45

(Drelon et al., 2019)

wing disc | third instar larval stage

(Drelon et al., 2018)

wing disc | third instar larval stage | conditional

(Drelon et al., 2018)

wing pouch | wandering third instar larval stage

(Drelon et al., 2019)

Detailed Description

Statement

Reference

Kdm5¹⁴⁰ homozygotes show a slow-growth phenotype that results in larvae taking longer to develop to the same size as controls animals. The third instar larval muscle exhibits altered NMJ morphology, with decreased number and increased size of type Ib synaptic boutons, which present unbundled microtubules.

In Kdm5¹⁴⁰ homozygous third instar larvae bearing two copies of Kdm5^JmjC.Tag:HA, muscles exhibit altered NMJ morphology and physiology, with decreased number of synaptic boutons and decreased eEJC amplitudes. These larvae show decreased crawling ability (decreased crawling length, decreased average and maximum crawling speed, and decreased speed normalized to body length).

In Kdm5¹⁴⁰ homozygous third instar larvae bearing two copies of Kdm5^L854F.Tag:HA, muscles exhibit altered NMJ morphology, with decreased number and increased size of type Ib synaptic boutons, which present unbundled microtubules. These larvae show decreased crawling ability (decreased crawling length, decreased average and maximum crawling speed, and decreased speed normalized to body length).

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ homozygotes exhibit developmental delay compared to controls.

Kdm5¹⁴⁰ wandering third instar larvae exhibit significantly decreased number of dividing cells (PH3+) and significantly increased cell death (Dcp-1+) in the wing pouches compared to controls.

Kdm5¹⁴⁰ third instar larvae exhibit a significant decrease in the number of prothoracic gland cells undergoing S phase of endocycling compared to controls.

(Drelon et al., 2019)

lid¹⁴⁰ mutants are lethal; pharate adults have morphologically normal heads, thoraces, legs, and abdominal segmentation, but fail to eclose.

lid¹⁴⁰ homozygotes show a severe delay to pupariation, a significant decrease in larval weight, but do not show defects in food intake, as compared to controls. Despite taking 5 days longer, the final larval, imaginal disc, and pupal size are normal; the pupal wing shows similar growth of the axon along the L3 wing vein, in addition to the correct number and position of the sensory bristles along the anterior margin of the wing, as compared to controls.

Larval wings disc are significantly smaller at 5.5 after egg laying, exhibit a significant decrease in cell division (mitotic index/phospho-H3) and exhibit a significant increase in apoptosis (Dcp-1-positive cells). Upon irradiation, the wing discs show increased apoptosis, as compared to irradiated controls (Dcp-1 immunostaining levels).

(Drelon et al., 2018)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Kdm5¹⁴⁰ has abnormal developmental rate phenotype, suppressible by Scer\GAL4^spok.1.45/tor^UAS.Tag:V5

(Drelon et al., 2019)

Kdm5¹⁴⁰ has abnormal developmental rate phenotype, suppressible by Scer\GAL4^spok.1.45/Ras85D^V12.cUa.UAS

(Drelon et al., 2019)

Kdm5¹⁴⁰ has increased mortality during development phenotype, suppressible by Scer\GAL4^spok.1.45/tor^UAS.Tag:V5

(Drelon et al., 2019)

Kdm5¹⁴⁰ has increased mortality during development phenotype, suppressible by Scer\GAL4^spok.1.45/Ras85D^V12.cUa.UAS

(Drelon et al., 2019)

Kdm5¹⁴⁰ has abnormal endomitotic cell cycle | third instar larval stage phenotype, suppressible by Scer\GAL4^spok.1.45/tor^UAS.Tag:V5

(Drelon et al., 2019)

Kdm5¹⁴⁰ has abnormal endomitotic cell cycle | third instar larval stage phenotype, suppressible by Scer\GAL4^spok.1.45/CycE^UAS.cRa

(Drelon et al., 2019)

Kdm5¹⁴⁰ has abnormal developmental rate phenotype, suppressible by Scer\GAL4^spok.1.45/CycE^UAS.cRa

(Drelon et al., 2019)

NOT suppressed by

Statement

Reference

Kdm5¹⁴⁰ has lethal phenotype, non-suppressible by Scer\GAL4^spok.1.45/CycE^UAS.cRa

(Drelon et al., 2019)

Other

Statement

Reference

Kdm5¹⁴⁰, Ras85D^V12.cUa.UAS, Scer\GAL4^spok.1.45 has visible | adult stage phenotype

(Drelon et al., 2019)

Kdm5¹⁴⁰, Scer\GAL4^spok.1.45, tor^UAS.Tag:V5 has visible | adult stage phenotype

(Drelon et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

Kdm5¹⁴⁰ has prothoracic gland | third instar larval stage phenotype, suppressible by Scer\GAL4^spok.1.45/tor^UAS.Tag:V5

(Drelon et al., 2019)

Kdm5¹⁴⁰ has prothoracic gland | third instar larval stage phenotype, suppressible by Scer\GAL4^spok.1.45/CycE^UAS.cRa

(Drelon et al., 2019)

Other

Statement

Reference

Kdm5¹⁴⁰, Ras85D^V12.cUa.UAS, Scer\GAL4^spok.1.45 has wing phenotype

(Drelon et al., 2019)

Kdm5¹⁴⁰, Scer\GAL4^spok.1.45, tor^UAS.Tag:V5 has wing phenotype

(Drelon et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

FBal0366860:, Kdm5¹⁴⁰ is rescued by Kdm5^L854F.Tag:HA/Kdm5^L854F.Tag:HA

(Belalcazar et al., 2021)

FBal0325457:, Kdm5¹⁴⁰ is rescued by Kdm5^Tag:HA/Kdm5^Tag:HA

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is rescued by Scer\GAL4^elav-C155/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is rescued by Scer\GAL4^da.G32/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is rescued by Scer\GAL4^RapGAP1-OK6/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is rescued by Kdm5^Tag:HA

(Drelon et al., 2018)

Partially rescued by

FBal0366861:, Kdm5¹⁴⁰ is partially rescued by Kdm5^R873W.Tag:HA/Kdm5^R873W.Tag:HA

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^Act5C.PI/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

FBal0366862:, Kdm5¹⁴⁰ is partially rescued by Kdm5^Y874C.Tag:HA/Kdm5^Y874C.Tag:HA

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^Ubi.PU/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^32B/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^Mai60/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^phtm.PO/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Kdm5^UASp.cSa/Scer\GAL4^T80

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Scer\GAL4^spok.1.45/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is partially rescued by Kdm5^{W1771A.Tag:HA}

(Drelon et al., 2018)

Not rescued by

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Mef2.PR/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^repo/Kdm5^UASp.cSa

(Belalcazar et al., 2021)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Cg.PA/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Ilp2.PR/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Aug21/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Mef2.PR/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Akh.PL/Kdm5^UASp.cSa

(Drelon et al., 2019)

Kdm5¹⁴⁰ is not rescued by Scer\GAL4^Toll-6-D42/Kdm5^UASp.cSa

(Drelon et al., 2019)

Comments

Expression of Kdm5^UASp.cSa under the control of Scer\GAL4^32B, Scer\GAL4^Mai60, Scer\GAL4^spok.1.45 or Scer\GAL4^phm.PO in the Kdm5¹⁴⁰ homozygous background leads to curved-down wings compared to controls.

(Drelon et al., 2019)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Kdm5¹⁴⁰

(Yheskel et al., 2024)

Kdm5^Δ

(Yheskel et al., 2024)

kdm5¹⁴⁰

(Yheskel et al., 2023, Belalcazar et al., 2021, Hatch et al., 2021, Drelon et al., 2019, Drelon et al., 2018, Zamurrad et al., 2018)

lid¹⁴⁰

(Voortman et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (8)

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