A TI{CRIMIC.GT14} DNA cassette has been inserted into unc-119, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The insertion is also within CG1677, not in a coding intron, and it is not clear whether or not it acts to gene trap this gene. The insertion is also within lncRNA:CR44357, not in a coding intron, and it is not clear whether or not it acts to gene trap this gene. The TI{CRIMIC.GT14} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CTGTGAGATGAGGCTTCTATTGG. The homology arms of the donor vector overlapped by 2 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.

The PCR check of the insertion gave no product for either flank.