A TI{CRIMIC.TG4.0} DNA cassette has been inserted into cta, in a coding intron, and is predicted to gene trap all annotated transcripts of the gene. The TI{CRIMIC.TG4.0} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: TACATATGGAACCACGGTTCGGG. The homology arms of the donor vector overlapped by 2 bp. It is unknown whether the insertion is precise or if there is a small deletion or duplication. The insertion site coordinate is the Cas9 cut site predicted for the CRISPR guide RNA.

The PCR check of the insertion gave a shorter than expected sized product for the left flank, but no product for the right flank.