FlyBase Allele Report: Dmel\Marf1[null]

General Information

Symbol

Dmel\Marf1^null

Species

D. melanogaster

Name

FlyBase ID

FBal0343501

Feature type

allele

Associated gene

Dmel\Marf1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

loss of function allele

(Zhu et al., 2018)

Mutagen

CRISPR/Cas9

(Zhu et al., 2018)

Progenitor genotype

Cytology

Description

241bp deletion that is predicted to result in a truncated peptide of 103 amino acid residues (revision of data in FBrf0240268).

(Fukunaga, 2019.2.4)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:22,363,681..22,363,921 [+]

Comment:

A 241bp deletion near the N-terminal end of the Marf1 coding sequence produces a frameshift and early translation termination.

(Fukunaga, 2019.2.4)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Zhu et al., 2018)

male fertile | recessive

(Zhu et al., 2018)

viable

(Zhu et al., 2018)

Phenotype Manifest In

germ plasm | oogenesis stage S14

(Zhu et al., 2018)

yolk granule | oogenesis stage S14

(Zhu et al., 2018)

Detailed Description

Statement

Reference

Marf1^null homozygous females are sterile as no egg hatches, despite the same number of eggs being laid and fertilized as in controls. Although ovaries and early stage oocytes display grossly normal morphologies and stage 14 oocytes exhibit proper metaphase I arrest, stage 14 oocytes show abnormal yolk aggregation and fail to localize germ plasm components to the oocyte posterior, and unfertilized eggs fail to show meiotic spindles or polar bodies.

(Zhu et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Marf1^null is rescued by Scer\GAL4^{VP16.mat.αTub67C}/Marf1^UASp.Tag:HA

(Zhu et al., 2018)

Marf1^null is rescued by Scer\GAL4^{VP16.mat.αTub67C}/Marf1^UASp.EGFP

(Zhu et al., 2018)

Not rescued by

Marf1^null is not rescued by Marf1^1-340.UASp/Scer\GAL4^{VP16.mat.αTub67C}

(Zhu et al., 2018)

Marf1^null is not rescued by Marf1^1-411.UASp/Scer\GAL4^{VP16.mat.αTub67C}

(Zhu et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG17018^null

(Zhu et al., 2018)

MARF1^null

(Zhu et al., 2018)

Marf1^null

(Fukunaga, 2019.2.4)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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