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General Information
Symbol
Scer\GAL4dsx-GAL4(DBD)::Zip-
Species
S. cerevisiae
Name
FlyBase ID
FBal0344075
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain) has been inserted at the start codon within exon 2 of dsx. This results in the hemidriver being expressed under the control of endogenous dsx sequences, along with deletion of exon 2.

Insertion components
TI{GAL4(DBD)::Zip-}dsxGAL4(DBD)::Zip-
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult doublesex TN1A (male) neuron

Comment: when combined with P{VT017258-p65.AD} (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

adult doublesex msB TN2 (male) neuron

Comment: when combined with P{VT017258-p65.AD} (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\TeTxLCFRT.stop.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males results in much less sine song during courtship than controls, but a similar amount of pulse song, with similar inter-pulse interval, carrier frequency and amplitude to controls.

Acute optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with 660 nm (red) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males, in the absence of females, does not significantly induce courtship song.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Scer\GAL4dsx-GAL4(DBD)::Zip-
Name Synonyms
Secondary FlyBase IDs
    References (2)