FB2025_05 , released December 11, 2025
Allele: Hsap\RELAAD.VT017258
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General Information
Symbol
Hsap\RELAAD.VT017258
Species
H. sapiens
Name
FlyBase ID
FBal0344078
Feature type
allele
Associated gene
Hsap\RELA
Associated Insertion(s)
Carried in Construct
P{VT017258-p65.AD}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Shirangi et al., 2016)
Progenitor genotype
Carried in construct
P{VT017258-p65.AD}
Cytology
Description

The VT017258 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Shirangi et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
VT017258
Encoded product / tool
p65(AD)::Zip+
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult doublesex TN1A (male) neuron

      Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4(DBD)::Zip- (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

      (Shirangi et al., 2016)
      adult doublesex msB TN2 (male) neuron

      Comment: when combined with TI{GAL4(DBD)::Zip-}dsxGAL4(DBD)::Zip- (combination referred to as 'VT017258- p65AD::Zp ∩ dsx[ Zp::Gdbd ]')

      (Shirangi et al., 2016)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Inhibition (using Ctet\TeTxLCFRT.stop.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males results in much less sine song during courtship than controls, but a similar amount of pulse song, with similar inter-pulse interval, carrier frequency and amplitude to controls.

      Acute optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with 660 nm (red) light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4dsx-GAL4(DBD)::Zip- and Hsap\RELAAD.VT017258 expressing Scer\FLP1L.8xlexAop under the control of Ecol\lexAGMR24B02 in males, in the absence of females, does not significantly induce courtship song.

      (Shirangi et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hsap\RELAAD.VT017258
      Name Synonyms
      Secondary FlyBase IDs
        References (2)