FB2025_01 , released February 20, 2025
Allele: Scer\GAL4DBD.Rab11.6.14.3
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General Information
Symbol
Scer\GAL4DBD.Rab11.6.14.3
Species
S. cerevisiae
Name
FlyBase ID
FBal0344508
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{Rab11-GAL4.DBD.6.14.3}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Hu et al., 2017)
Progenitor genotype
Carried in construct
P{Rab11-GAL4.DBD.6.14.3}
Cytology
Description

Rab11 1.3kb enhancer fragment downstream of the original P{IT.GAL4}Rab116.14-G4 insertion regulates expression of a GAL4(DBD)::Zip- split driver.

(Hu et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
Rab11
Encoded product / tool
GAL4(DBD)::Zip-
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage
      larval A08n neuron

      Comment: when combined with P{R82E12-p65.AD}

      (Hu et al., 2017)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Hyperpolarization (using Hsap\KCNJ2UAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Rab11.6.14.3 and Hsap\RELAAD.R82E12 in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; there is no effect on the response to gentle touch or locomotion speed, compared to controls.

      Inhibition (using Ctet\tetXTNT-LC.UAS) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Rab11.6.14.3 and Hsap\RELAAD.R82E12 in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, but does not affect the latency of rolling in response to high temperature (local) stimulation, compared to controls.

      Optogenetic activation (using Cnoc\ChR1::Csub\ChRCsChrimson.IVS.20xUAS.Venus with 625 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.Rab11.6.14.3 and Hsap\RELAAD.R82E12 in larvae significantly increases rolling responses in the absence of pain stimuli, compared to controls.

      (Hu et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Scer\GAL4DBD.Rab11.6.14.3
      Name Synonyms
      Secondary FlyBase IDs
        References (5)