FlyBase Allele Report: Dmel\sov[RNAi.UAS.3-2]

General Information

Symbol

Dmel\sov^RNAi.UAS.3-2

Species

D. melanogaster

Name

FlyBase ID

FBal0344869

Feature type

allele

Associated gene

Dmel\sov

Associated Insertion(s)

Carried in Construct

P{UAS-sov.RNAi.3-2}

Key Links

Transgenic product class

RNAi_reagent

(Jankovics et al., 2018)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Jankovics et al., 2018)

Mutagen

in vitro construct

(Jankovics et al., 2018)

Progenitor genotype

Carried in construct

P{UAS-sov.RNAi.3-2}

Cytology

Description

UAS regulates expression of a shRNA targeting the last exon of sov.

(Jankovics et al., 2018)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

sov

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal axis specification, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

decreased cell number | oogenesis, with Scer\GAL4^C587

(Jankovics et al., 2018)

decreased cell number | oogenesis | progressive, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

female sterile, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

neoplasia | oogenesis, with Scer\GAL4^C587

(Jankovics et al., 2018)

visible | egg stage, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

Phenotype Manifest In

cystoblast, with Scer\GAL4^C587

(Jankovics et al., 2018)

dorsal appendage, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

egg, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

escort cell, with Scer\GAL4^C587

(Jankovics et al., 2018)

female germline stem cell, with Scer\GAL4^C587

(Jankovics et al., 2018)

female germline stem cell, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

germarium, with Scer\GAL4^C587

(Jankovics et al., 2018)

germarium, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

oocyte, with Scer\GAL4^nanos.PG

(Jankovics et al., 2018)

Detailed Description

Statement

Reference

Females expressing sov^{dsRNA.UAS.3-2} under the control of Scer\GAL4^nos.PG are sterile; young females show normal-looking ovaries, but their oocytes and eggs show axis-specification defects (i.e. mislocalization of osk mRNA and of Osk, Grk and Vasa proteins in the oocyte; ventralized eggshells, with fused or absent dorsal appendages) and eggs do not hatch. These females show a progressive decrease in germline stem cells in the germarium, as 4-week old germaria lack germline stem cells whereas 1-week old do not, as compared to controls.

Expressing sov^{dsRNA.UAS.3-2} under the control of Scer\GAL4^C587 results in most germaria showing no germ stem cells while almost all remaining germaria showing germ cell tumors, as well as a severe decrease in escort cells, namely in the niche. Adulthood-only expression (by using Gal80[ts]) show similar changes in germ cells in the germarium.

(Jankovics et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^C587, sov^RNAi.UAS.3-2 has decreased cell number | oogenesis phenotype, non-suppressible by Scer\GAL4^C587/Wnt4^{UAS.ORF.Tag:HA}

(Jankovics et al., 2018)

Scer\GAL4^C587, sov^RNAi.UAS.3-2 has neoplasia | oogenesis phenotype, non-suppressible by Scer\GAL4^C587/Wnt4^{UAS.ORF.Tag:HA}