Reported as a 604bp deletion that removes the Ptth ATG and signal sequence. The approximate mapped boundaries of the deletion were inferred from the reported primer sequences and extend 600 bases. This region was replaced with sequences from a pTV-Cherry-based donor plasmid.
The deletion was reported as a 604bp deletion but based on the primers used, maps to 2L:576323..576922 , a 600bp deletion. The deleted region was replaced with sequences from a pTV-Cherry-based donor plasmid.
Part of Ptth, including the putative ATG codon and the signal peptide, has been deleted. The following sequence (originating from the pTV-Cherry-based donor plasmid) has been inserted in place of the deleted Ptth sequence: a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w+* marker.