UAS regulates expression of a human KIF5B-RET fusion gene cDNA, derived from a non-small-cell lung cancer (NSCLC) patient, in which the Motor Domain (MD) and Coiled Coil (CC) domain of Hsap\KIF5B is fused to the kinase (K) domain of Hsap\RET.
Expressing Hsap\KIF5B::Hsap\RETUAS.cDa under the control of Scer\GAL4ptc.PU is nearly pupal lethal and the few adult escapers exhibit ectopic wing veins. Expression under the control of Scer\GAL4C-765 leads also to ectopic wing veins.
Wing disc epithelial cells expressing Hsap\KIF5B::Hsap\RETUAS.cDa under the control of Scer\GAL4ptc.PU shift basally, show initial signs of cell polarity defects (E-cadherin, which is normally present primarily in the apical regions of epithelia, is delocalized), and extend long processes into the neighboring region of non-expressing cells, a phenomenon not observed in controls; these cells are associated with a severely degraded basal lamina.
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has visible | adult stage phenotype, suppressible by EgfrJF02283, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab21JF03338, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab23HMC04039, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by GripHMS02178, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Arp3HMS00711, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by CortactinHMS00658, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Src64BHMC03327, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by PvrHMS01662, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by RetHMC03102, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by btlHMS02038, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by htlHMS04514, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by InRHMS03166, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by GripJF02969, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has abnormal cell polarity | third instar larval stage phenotype, suppressible | partially by GripJF02969, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab6HMS01486, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab7JF02377, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab8HMS01363, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has increased mortality during development phenotype, suppressible | partially by Rab9HMS02635, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has lethal - all die before end of P-stage phenotype, non-suppressible by Rab11JF02812, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has lethal - all die before end of P-stage phenotype, non-suppressible by Rab5HMS00147, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has wing vein | ectopic phenotype, suppressible by EgfrJF02283, Scer\GAL4ptc.PU
Hsap\KIF5B::Hsap\RETUAS.cDa, Scer\GAL4ptc.PU has wing disc | third instar larval stage phenotype, suppressible | partially by GripJF02969, Scer\GAL4ptc.PU