FB2025_01 , released February 20, 2025
Allele: Dmel\Polr2Ahuman.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Polr2Ahuman.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0347219
Feature type
allele
Associated gene
Dmel\Polr2A
Associated Insertion(s)
Carried in Construct
M{UAS-Polr2A.human.FLAG}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Portz et al., 2017)
Progenitor genotype
Carried in construct
M{UAS-Polr2A.human.FLAG}
Cytology
Description

UASt regulatory sequences drive expression of RpII215 in which the C-terminal domain (CTD) has been replaced with the CTD from the orthologous human gene. The coding sequence is tagged at the C-terminal end with two copies of Tag:FLAG and has been rendered resistant to RNAi by the P{TRiP.GL01068} line by synonymous mutations in the sequence targeted by the P{TRiP.GL01068} shRNA (AACGGTGAAACTGTCGAACAA mutated to AACCGTCAAGTTGAGCAACAA).

(Portz et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Polr2A
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Polr2Ahuman.UAS.Tag:FLAG
      RpII215human.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)