FB2020_06, released Dec 22, 2020

Allele: Dmel\larp4D.UASp.GFP,Tag:Mito(Tom20)

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General Information
Symbol
Dmel\larp4D.UASp.GFP,Tag:Mito(Tom20)
Species
D. melanogaster
Name
FlyBase ID
FBal0347464
Feature type
allele
Associated gene
Dmel\larp
Associated Insertion(s)
Carried in Construct
P{UASp-Tom20-larp.4D.GFP}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Zhang et al., 2019)
in vitro construct - coding region fusion
(Zhang et al., 2019)
in vitro construct - amino acid replacement
(Zhang et al., 2019)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UASp-Tom20-larp.4D.GFP}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASp regulatory sequences drive expression of larp that has been mutated to carry the phosphomimetic amino acid replacement S1119D. The protein is targeted to the mitochondrial outer membrane by fusion with Tom20 and is also tagged with GFP.

      (Zhang et al., 2019)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASp
      Encoded product / tool
      larp
      Tagged with
      GFP
      Tag:Mito(Tom20)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Females expressing larp4D.UASp.GFP,Tag:Mito(Tom20) under the control of Scer\GAL4nos.PU lay eggs that lack the counterselection ability of mitochondrial temperature sensitive (mt-ts, mt:CoIT300I) genome in a heteroplasmic background containing both mt-ts and wild-type mitochondrial genomes at a restrictive temperature (29[o]C) different than controls.

      (Zhang et al., 2019)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      larp4D.UASp.GFP,Tag:Mito(Tom20)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)