UASt regulatory sequences drive expression of the 17 C-terminal amino acids of brp, tagged at the N-terminal end with 3xTag:FLAG and CFP.
abnormal locomotor behavior | larval stage, with Scer\GAL4RapGAP1-OK6
abnormal locomotor behavior | larval stage, with Scer\GAL4VGlut.PD
abnormal neurophysiology | larval stage, with Scer\GAL4RapGAP1-OK6
A1-7 ventral longitudinal muscle 3, with Scer\GAL4RapGAP1-OK6
Expression of brpC-tip.UAS.CFP,Tag:FLAG under the control of Scer\GAL4RapGAP1-OK6 or Scer\GAL4VGlut.PD in larvae significantly reduces locomotion, compared to controls; expression under the control of Scer\GAL4RapGAP1-OK6 additionally significantly increases paired-pulse depression of excitatory postsynaptic currents (eEPSCs) in muscle 6, compared to controls.
brpC-tip.UAS.CFP,Tag:FLAG/Scer\GAL4RapGAP1-OK6 rescues brpC-long.UAS.Tag:M(mCd8a),EGFP