FlyBase Allele Report: Hsap\ABL1::Hsap\BCR[UAS.cBa]

General Information

Symbol

Hsap\ABL1::Hsap\BCR^UAS.cBa

Species

H. sapiens

Name

UAS construct a of Bernardoni

FlyBase ID

FBal0348146

Feature type

allele

Associated gene

Hsap\ABL1::Hsap\BCR

Associated Insertion(s)

Carried in Construct

P{UAS-BCR-ABL1}

Also Known As

UAS-BCR-ABL1

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Bernardoni et al., 2019)

Progenitor genotype

Carried in construct

P{UAS-BCR-ABL1}

Cytology

Description

UAS regulatory sequences drive expression of the constitutively active, oncogenic Hsap\ABL1::Hsap\BCR fusion protein that is associated with chronic myeloid leukemia.

(Bernardoni et al., 2019)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\ABL1::Hsap\BCR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of chronic myeloid leukemia

CEA

(Bernardoni et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of chronic myeloid leukemia

is ameliorated by Stat92E^ΔN.UAS

(Bernardoni et al., 2019)

is ameliorated by Stat92E⁰⁶³⁴⁶

(Bernardoni et al., 2019)

is ameliorated by ena²³

(Bernardoni et al., 2019)

is ameliorated by ena^GD8910

(Bernardoni et al., 2019)

is ameliorated by ena^KK107752

(Bernardoni et al., 2019)

is exacerbated by fax^M7

(Bernardoni et al., 2019)

is exacerbated by pros¹⁷

(Bernardoni et al., 2019)

is ameliorated by Abl^GD1344

(Bernardoni et al., 2019)

is ameliorated by Abl^K417N.UAS

(Bernardoni et al., 2019)

is exacerbated by Abl^UAS.cFa

(Bernardoni et al., 2019)

is exacerbated by Dab^GD4886

(Bernardoni et al., 2019)

is ameliorated by Dab^UAS.cUa

(Bernardoni et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Bernardoni et al., 2019)

BCR/ABL1_fusion

(FlyBase curators, 2019-)

Variants Synonym(s)

Associated human disease model(s)

chronic myeloid leukemia, BCR-ABL1 fusions

External database links

COSMIC_fusion: BCR-ABL1

Comments concerning this variant

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hemocyte | third instar larval stage, with Scer\GAL4^dome.PU

(Bernardoni et al., 2019)

eye, with Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

eye, with Scer\GAL4^sev.PU

(Bernardoni et al., 2019)

eye disc posterior to the morphogenetic furrow | third instar larval stage, with Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

interommatidial bristle | ectopic, with Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Detailed Description

Statement

Reference

Expressing Hsap\ABL1::Hsap\BCR^UAS.cBa under the control of Scer\GAL4^sev.PU or Scer\GAL4^GMR.PU induces a rough eye phenotype that is mild or severe, respectively. With Scer\GAL4^GMR.PU-driven expression, the eye is small, bar-shaped and "glazed", with depigmented areas, and misplaced extra sensory bristles appearing in the dorsal region; in agreement, the typical Elav-positive photoreceptor clusters in the third instar larval eye disc are reduced in number and altered.

Expression of Hsap\ABL1::Hsap\BCR^UAS.cBa under the control of Scer\GAL4^dome.PU, either consititutively or induced from L1 onwards (under the additional control of Gal80[ts] and temperature shift to 29[o]C) induces lethality. Induced expression form L2 onwards, but not L3 onwards, leads to the development of melanotic masses at L3, which correlates with a severe increase in the number of circulating hemocytes.

(Bernardoni et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Abl^UAS.cFa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Dab^GD4886, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, enhanceable by Dab^GD4886, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has decreased cell number | third instar larval stage phenotype, enhanceable by Dab^GD4886, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Df(3R)T-32/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Df(3L)st-f13/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, enhanceable by Abl^UAS.cFa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by Df(3L)81k19/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^dome.PU has melanotic mass phenotype | third instar larval stage phenotype, enhanceable by Dab^GD4886, Scer\GAL4^dome.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by pros[+]/pros¹⁷

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, enhanceable by fax^M7/fax[+]

(Bernardoni et al., 2019)

NOT Enhanced by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-enhanceable by Dab[+]/Dab^EY10190

(Bernardoni et al., 2019)

Suppressed by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by Abl¹/Abl[+]

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Abl^K417N.UAS, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Abl^GD1344, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by ena²³/ena[+]

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by ena^GD8910, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by ena^KK107752, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, suppressible | partially by ena^GD8910, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has abnormal size | adult stage phenotype, suppressible | partially by ena^KK107752, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has decreased cell number | third instar larval stage phenotype, suppressible | partially by ena^GD8910, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Stat92E^ΔN.UAS, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^dome.PU has melanotic mass phenotype | third instar larval stage phenotype, suppressible | partially by Abl^GD1344, Scer\GAL4^dome.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^dome.PU has melanotic mass phenotype | third instar larval stage phenotype, suppressible by Dab^UAS.cUa, Scer\GAL4^dome.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^dome.PU has melanotic mass phenotype | third instar larval stage phenotype, suppressible | partially by ena^KK107752, Scer\GAL4^dome.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by Df(2R)P34/+

(Bernardoni et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has visible | adult stage phenotype, non-suppressible by Dab[+]/Dab^EY10190

(Bernardoni et al., 2019)

Enhancer of

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU is an enhancer of abnormal size | adult stage phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Abl^UAS.cFa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Dab^GD4886, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye disc posterior to the morphogenetic furrow | third instar larval stage phenotype, enhanceable by Dab^GD4886, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Df(3R)T-32/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Df(3L)st-f13/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by Df(3L)81k19/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by pros[+]/pros¹⁷

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, enhanceable by fax^M7/fax[+]

(Bernardoni et al., 2019)

NOT Enhanced by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, non-enhanceable by Dab[+]/Dab^EY10190

(Bernardoni et al., 2019)

Suppressed by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Stat92E^ΔN.UAS, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Df(2R)P34/+

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Abl¹/Abl[+]

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Abl^K417N.UAS, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by Abl^GD1344, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible by ena²³/ena[+]

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by ena^GD8910, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by ena^KK107752, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye disc posterior to the morphogenetic furrow | third instar larval stage phenotype, suppressible | partially by ena^GD8910, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, suppressible | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(Bernardoni et al., 2019)

NOT suppressed by

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU has eye phenotype, non-suppressible by Dab[+]/Dab^EY10190

(Bernardoni et al., 2019)

Enhancer of

Statement

Reference

Hsap\ABL1::Hsap\BCR^UAS.cBa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Abl^UAS.cFa, Scer\GAL4^GMR.PU

(Bernardoni et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Hsap\ABL1::Hsap\BCR^UAS.cBa

Name Synonyms

UAS construct a of Bernardoni

Secondary FlyBase IDs

References (3)

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