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General Information
Symbol
Dmel\Syt1DF-C.UAS.pHluorinSE.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0349056
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a sensor that is composed of Syt1 fused at its C-terminal end to mCherry and then pHluorinSE. The presence of Syt1 targets the protein to the synaptic vesicle membrane (the dual fluorophore faces the cytoplasm). The mCherry-pHluorinSE dual fluorophore can act as an acidification sensor in vivo as pHluorinSE is pH sensitive but mCherry is pH-resistant; red fluorescence indicates an acidic environment (pH < 6), while yellow fluorescence indicates a pH greater or equal to 7. In addition, the C-terminal pHluorinSE moiety is expected to be more exposed to proteases than mCherry, such that the dual fluorophore can also be used as a degradation sensor by observing fluorescence in fixed samples; yellow fluorescence indicates both fluorophores are intact, red fluorescence indicates degradation of the C-terminal pHluorinSE moiety.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Syt1DF-C.UAS.pHluorinSE.mCherry
Name Synonyms
Secondary FlyBase IDs
    References (1)