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General Information
Symbol
Dmel\mamoUAS.gRNAx2.pCFD6
Species
D. melanogaster
Name
FlyBase ID
FBal0350121
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of two sgRNAs (AGTACGAGGAACAAGCCGAG and GCAGTGAGCACTACTGCTTG), both of which target mamo. The two sgRNAs are expressed as a single polycistronic tRNA-gRNA cassette.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Crispr-Cas9 mutagenesis of mamo by expressing Spyo\Cas9UAS.Tag:NLS(Unk) under the control of Scer\GAL4GMR71G10 and using mamoUAS.gRNAx2.pCFD6 as a guide (also expressed under the control of Scer\GAL4GMR71G10) results in a high frequency of mushroom body gamma neuron pruning defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
mamoUAS.gRNAx2.pCFD6
Name Synonyms
Secondary FlyBase IDs
    References (1)