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General Information
Symbol
Dmel\Pol32L30
Species
D. melanogaster
Name
FlyBase ID
FBal0350363
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:
An 803 bp deletion resulting from the imprecise excision of P{EPgy2}Pol32EY15283. Reported to map to 2L:15255502..15256304. 39 bases of P element sequences remain at the site of the excision.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Mobilization of the progenitor P{EPgy2}Pol32EY15283 insertion, resulting in deletion of nt15255502 to nt15256304 with an addition of 39bp of P element sequences. Since only part of the Pol32 coding region is removed, along with the entire annotated 3'UTR, there is still a possibility that a truncated Pol32 protein with 219 residues is produced, though this is undetectable by Western Blot analyses.
Insertion components
P{?EPgy2}Pol32L30
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Pol32L30 mutant adults express a variable degree of bristle loss or shortening, as compared to controls. Females are sterile, as they lay eggs that are fertilized but do not show more than 8 nuclei; in agreement, most of the DNA from these embryos is mitochondrial. A small proportion of Pol32L30/Pol32L27 third instar larvae show double strand breaks, which are nearly absent in controls. A very small proportion of adults show an etched abdomen phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference
A small proportion of Pol32L30/Pol32L27 third instar larvae show double strand breaks, both centric and non-centric, which are nearly absent in controls; these become much more frequent in a DNApol-δI10 heterozygous background and are both centric and non-centric. A very small proportion of Pol32L30/Pol32L27 adults show an etched abdomen phenotype; this phenotype is only slightly increased in a DNApol-α180G13925/+ background, is moderately increased in a Pol31G16501/+ background, becomes highly prevalent in Df(3L)BSC122/+ or Df(3R)Exel6186/+ backgrounds and becomes nearly fully penetrant in a DNApol-δI10/+ background; the phenotype is not much affected in Df(3R)X3F/+, spn-A1/+, Df(3R)P-21/+, or BlmD2/+ backgrounds.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pol32L30
pol32L30
Name Synonyms
Secondary FlyBase IDs
    References (1)