FB2025_01 , released February 20, 2025
Allele: Dmel\Cul4KR.UAS.Tag:FLAG
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General Information
Symbol
Dmel\Cul4KR.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0351282
Feature type
allele
Associated gene
Dmel\Cul4
Associated Insertion(s)
Carried in Construct
P{UAS-Cul4.KR.Flag}
Key Links
Transgenic product class
dominant_negative_variant
(Ly et al., 2019)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Ly et al., 2019)
Mutagen
in vitro construct
(Ly et al., 2019)
Progenitor genotype
Carried in construct
P{UAS-Cul4.KR.Flag}
Cytology
Description

UAS regulatory sequences drive expression of a dominant-negative form of Cul4; the neddylation site has been mutated. The coding sequence is is tagged with Tag:FLAG.

(Ly et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cul4
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Cul4KR.UAS.Tag:FLAG expression driven by Scer\GAL4insc-Mz1407 leads to larval brain neuroblasts showing signs of quiescence: neuroblasts show a significant decrease in cell divisions (both DNA replication/EdU staining and Mitosis/phospho-H3); and frequently show a primary cellular process, whereas control neuroblasts show a typically rounded morphology.

      (Ly et al., 2019)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Cul4KR.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (1)